folE

GTP cyclohydrolase I, required for the initial step of queuosine synthesis ([SW|tRNA modification])

locus: BSU_22780

pI: 6.34

mw: 21.07 kDa

protein length: 190 aa

gene length: 573 bp

function: biosynthesis of folate and queuosine

product: GTP cyclohydrolase IA

essential: yes

ec: 3.5.4.16

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.5|Biosynthesis of folate]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,384,783 2,385,355

Phenotypes of a mutant

• essential [Pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• GTP + H₂O --> 7,8-dihydroneopterin 3'-triphosphate + formate + H⁺ (according to UniProt)

Protein family

• GTP cyclohydrolase I family (with [protein|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|QueF], according to UniProt)

[SW|Cofactors]

• zinc-dependent enzyme [Pubmed|19767425]

Effectors of protein activity

• the interaction with ZagA/ZTP facilitates the supply of zinc to FolE under conditions of zinc limitation, and thus enzymatic activity [pubmed|31132310]

Structure

• [PDB|3D2O] (enzyme from ''Neisseria gonorrhoeae'') [Pubmed|17032654]

Expression and Regulation

Operons

• genes: [gene|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE]-[gene|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]
  description: [Pubmed|2123343]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2123343], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • constitutive [Pubmed|19767425]

References

Reviews

• 31220391

Original publications

• 1551827,2123343,17032654,19767425,28189581,31132310