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hprK [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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hprK [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

HPr kinase/ phosphorylase
locus
BSU35000
pI
4.00
mw
34.00 kDa
function
carbon catabolite repression,
phosphorylation of HPr and Crh proteins at Ser46
product
HPr kinase/ phosphorylase
essential
no
synonyms
ptsK, yvoB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]

    • [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|transcription factors and their control]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,594,242 -> 3,595,174

    Phenotypes of a mutant

  • no carbon catabolite repression [Pubmed|9570401]
  • delay in spore [SW|germination] [Pubmed|26381121]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP [ptsH|search|HPr] = ADP P-Ser-HPr (according to Swiss-Prot)

    • Protein family

  • HPrK/P family (according to Swiss-Prot)

    • Structure

  • [PDB|1KKM] (complex of Lactobacillus casei HprK with B. subtilis HPr-Ser-P), [PDB|1KKL] (complex of Lactobacillus casei HprK with B. subtilis HPr)

    • [SW|Interactions]

  • HprK-[PtsH|search|HPr]
  • HprK-[protein|search|Crh] [Pubmed|12009882]

    • Expression and Regulation

    Operon

  • ''[protein|search|hprK]-[protein|search|lgt]-[protein|search|yvoD]-[protein|search|yvoE]-[protein|search|yvoF]'' [Pubmed|9465101]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|23667565]

    • Biological materials

    Mutant

  • GP202 (spc) [Pubmed|12055300], GP858 (aphA3) [Pubmed|18757537], GP82 (cat) [Pubmed|21992469], all available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP642, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification of mutant HprK-G158A from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP650, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP205, available in [SW|Jörg Stülke]'s lab
  • for expression, purification of the N-terminal in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP218, available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP201 (in [protein|search|pAC5], [Pubmed|12055300]) available in [SW|Stülke] lab, pGP202 (in [protein|search|pAC6], [Pubmed|12055300]) available in [SW|Jörg Stülke]'s lab

    • Antibody

  • available in [SW|Jörg Stülke]'s lab [Pubmed|12055300]

    • Labs working on this gene/protein

  • [SW|Josef Deutscher], Paris-Grignon, France
  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • [SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
  • [SW|Anne Galinier], University of Marseille, France

    • References

    Reviews

  • 12837773,15023355,18628769

    • General Analysis, Physiology

  • 9570401,9465101,12123463,18757537,22001508,23667565,26381121

    • Structural Analysis of HPrK

  • 11904409,12589763,12359875,17878158

    • Enzymatic Properties, Mutation Analysis

  • 12359880,11483496,12055300,10636874,12411438,11796714,12009882,12779331

    • HprK as a Target For Antimicrobial Compounds

  • 15084125