HPr kinase/ phosphorylase
function
carbon catabolite repression,
phosphorylation of HPr and Crh proteins at Ser46
product
HPr kinase/ phosphorylase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)][SW|Categories] containing this gene/protein
[SW|protein modification], [SW|transcription factors and their control]Gene
Coordinates on the chromosome (coding sequence)
3,594,242 -> 3,595,174
Phenotypes of a mutant
no carbon catabolite repression [Pubmed|9570401]delay in spore [SW|germination] [Pubmed|26381121]The protein
Catalyzed reaction/ biological activity
ATP [ptsH|search|HPr] = ADP P-Ser-HPr (according to Swiss-Prot)Protein family
HPrK/P family (according to Swiss-Prot)Structure
[PDB|1KKM] (complex of Lactobacillus casei HprK with B. subtilis HPr-Ser-P), [PDB|1KKL] (complex of Lactobacillus casei HprK with B. subtilis HPr)[SW|Interactions]
HprK-[PtsH|search|HPr]HprK-[protein|search|Crh] [Pubmed|12009882]Expression and Regulation
Operon
''[protein|search|hprK]-[protein|search|lgt]-[protein|search|yvoD]-[protein|search|yvoE]-[protein|search|yvoF]'' [Pubmed|9465101][SW|Sigma factor]
[protein|search|SigA] [Pubmed|23667565]Biological materials
Mutant
GP202 (spc) [Pubmed|12055300], GP858 (aphA3) [Pubmed|18757537], GP82 (cat) [Pubmed|21992469], all available in [SW|Jörg Stülke]'s labExpression vector
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP642, available in [SW|Jörg Stülke]'s labfor expression/ purification of mutant HprK-G158A from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP650, available in [SW|Jörg Stülke]'s labfor expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP205, available in [SW|Jörg Stülke]'s labfor expression, purification of the N-terminal in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP218, available in [SW|Jörg Stülke]'s lablacZ fusion
pGP201 (in [protein|search|pAC5], [Pubmed|12055300]) available in [SW|Stülke] lab, pGP202 (in [protein|search|pAC6], [Pubmed|12055300]) available in [SW|Jörg Stülke]'s labAntibody
available in [SW|Jörg Stülke]'s lab [Pubmed|12055300]Labs working on this gene/protein
[SW|Josef Deutscher], Paris-Grignon, France[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage][SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage][SW|Anne Galinier], University of Marseille, FranceReferences
Reviews
12837773,15023355,18628769 General Analysis, Physiology
9570401,9465101,12123463,18757537,22001508,23667565,26381121 Structural Analysis of HPrK
11904409,12589763,12359875,17878158 Enzymatic Properties, Mutation Analysis
12359880,11483496,12055300,10636874,12411438,11796714,12009882,12779331 HprK as a Target For Antimicrobial Compounds