hprK [2017-06-23 17:01:36]

HPr kinase/ phosphorylase

locus: BSU35000

pI: 4.91

mw: 34.55 kDa

protein length: 310 aa

gene length: 930 bp

function: carbon catabolite repression,phosphorylation of HPr and Crh proteins at Ser46

product: HPr kinase/ phosphorylase

essential: no

synonyms: ptsK, yvoB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]

Gene

Coordinates: 3,594,242 → 3,595,174

Phenotypes of a mutant

• no carbon catabolite repression [Pubmed|9570401]
• delay in spore [SW|germination] [Pubmed|26381121]

The protein

Catalyzed reaction/ biological activity

• ATP [gene|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]]] = ADP P-Ser-HPr (according to Swiss-Prot)

Protein family

• HPrK/P family (according to Swiss-Prot)

Structure

• [PDB|1KKM] (complex of Lactobacillus casei HprK with B. subtilis HPr-Ser-P), [PDB|1KKL] (complex of Lactobacillus casei HprK with B. subtilis HPr)

Expression and Regulation

Operons

• genes: [gene|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK]-[gene|2EB790AD1872ABCB57B784296C04C773AEA9C22B|lgt]-[gene|FA32AD908196BDA5C2C7C26EA08FB8416182CC3D|yvoD]-[gene|F3970DC380899E0505E80EB327AE27AFDE0F166F|yvoE]-[gene|C6B0EF2CD33D0F5455726167AE8F510619C3F6CA|yvoF]
  description: [Pubmed|9465101]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23667565], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-A328 (yvoB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/328 NBRP B. subtilis, Japan]
• GP202 (spc) [Pubmed|12055300], GP858 (aphA3) [Pubmed|18757537], GP82 (cat) [Pubmed|21992469], all available in [SW|Jörg Stülke]'s lab

Expression vector

• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP642, available in [SW|Jörg Stülke]'s lab
• for expression/ purification of mutant HprK-G158A from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP650, available in [SW|Jörg Stülke]'s lab
• for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP205, available in [SW|Jörg Stülke]'s lab
• for expression, purification of the N-terminal in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP218, available in [SW|Jörg Stülke]'s lab

lacZ fusion

• pGP201 (in [protein|search|pAC5], [Pubmed|12055300]) available in [SW|Stülke] lab, pGP202 (in [protein|search|pAC6], [Pubmed|12055300]) available in [SW|Jörg Stülke]'s lab

Antibody

• available in [SW|Jörg Stülke]'s lab [Pubmed|12055300]

Labs working on this gene/protein

• [SW|Josef Deutscher], Paris-Grignon, France
• [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
• [SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
• [SW|Anne Galinier], University of Marseille, France

References

Reviews

• 12837773,15023355,18628769

General Analysis, Physiology

• 9570401,9465101,12123463,18757537,22001508,23667565,26381121

Structural Analysis of HPrK

• 11904409,12589763,12359875,17878158

Enzymatic Properties, Mutation Analysis

• 12359880,11483496,12055300,10636874,12411438,11796714,12009882,12779331

HprK as a Target For Antimicrobial Compounds

• 15084125