SubtiBank SubtiBank
dacB
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dacB

sporulation-specific penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase
Locus
BSU_23190
Isoelectric point
9.73
Molecular weight
42.92 kDa
Protein length
382 aa Sequence Blast
Gene length
1149 bp Sequence Blast
Function
control of peptide cross-linking in spore peptidoglycan
Product
penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall synthesis → Penicillin-binding proteins
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,423,383 2,424,531

    Phenotypes of a mutant

  • inactivation of dacB reduces sporulation efficiency to 19.3% that of wild type cells PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • modifies degree of cross-linking of glycan strands in peptidoglycan PubMed
  • Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

    • Protein family

  • Peptidase S11 family (according to UniProt)

    • Paralogous protein(s)

  • DacF

    • Structure

  • 3MFD (beta-lactamase superfamily domain)

    • Localization

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    dacB-spmA-spmB
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
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    Biological materials

    Mutant

  • MGNA-A098 (dacB::erm), available at the NBRP B. subtilis, Japan
  • 1A744 ( dacB::cat), PubMed, available at BGSC
  • 1A745 ( dacB::cat), PubMed, available at BGSC
  • BKE23190 (dacB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG, downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC
  • BKK23190 (dacB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG, downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC

    • References

    Reviews

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    Original publications

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