iolA

methylmalonate-semialdehyde dehydrogenase (acylating)

Locus: BSU_39760

Isoelectric point: 5.14

Molecular weight: 53.29 kDa

Protein length: 487 aa

Gene length: 1464 bp

Function: myo-inositol catabolism

Product: methylmalonate-semialdehyde dehydrogenase (acylating)

Essential: no

E.C.: 1.2.1.27

Synonyms: mmsA,yxdA

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of inositol

Gene

Coordinates: 4,082,920 4,084,383

The protein

Catalyzed reaction/ biological activity

• NAD-dependent oxidation of methylmalonate semialdehyde (MMSA) to propionyl-CoA via acylation and deacylation steps
• 3-oxopropanoate + CoA + NAD⁺ --> acetyl-CoA + CO₂ + NADH (according to UniProt)
• 2-methyl-3-oxopropanoate + CoA + H₂O + NAD⁺ --> H⁺ + hydrogencarbonate + NADH + propanoyl-CoA (according to UniProt)

Protein family

• aldehyde dehydrogenase family (according to UniProt)

Paralogous protein(s)

• YwdH, GabD, YfmT, AldX, AldY, GbsA, PutC, YcbD, DhaS, RocA

Cofactors

• Coenzyme A, NAD PubMed

Structure

• 1T90 PubMed

Expression and Regulation

Operons

• Genes: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • IolR: repression, PubMed, in IolR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced by inositol (IolR) PubMed

Biological materials

Mutant

• MGNA-B770 (iolA::erm), available at the NBRP B. subtilis, Japan
• BKE39760 (iolA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA, downstream forward: _UP4_TAAACGACGAACAGCCGAAC
• BKK39760 (iolA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA, downstream forward: _UP4_TAAACGACGAACAGCCGAAC

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

Loading