iolA

methylmalonate-semialdehyde dehydrogenase (acylating)

locus: BSU_39760

pI: 5.14

mw: 53.29 kDa

protein length: 487 aa

gene length: 1464 bp

function: myo-inositol catabolism

product: methylmalonate-semialdehyde dehydrogenase (acylating)

essential: no

ec: 1.2.1.27

synonyms: mmsA,yxdA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.16|Utilization of inositol]

Gene

Coordinates: 4,082,920 4,084,383

The protein

Catalyzed reaction/ biological activity

• NAD-dependent oxidation of methylmalonate semialdehyde (MMSA) to propionyl-CoA via acylation and deacylation steps
• 3-oxopropanoate + CoA + NAD⁺ --> acetyl-CoA + CO₂ + NADH (according to UniProt)
• 2-methyl-3-oxopropanoate + CoA + H₂O + NAD⁺ --> H⁺ + hydrogencarbonate + NADH + propanoyl-CoA (according to UniProt)

Protein family

• [SW|aldehyde dehydrogenase family] (according to UniProt)

Paralogous protein(s)

• [protein|99F1FAF28FB4817D94E84BD5288FA33124558933|YwdH], [protein|A0BEB92D54799956A4ADE106A1388E5710141069|GabD], [protein|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|YfmT], [protein|F3341F205CB939498109D2A54DE842065C488DD5|AldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|AldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|GbsA], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|PutC], [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|YcbD], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|DhaS], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|RocA]

[SW|Cofactors]

• Coenzyme A, NAD [Pubmed|22782904]

Structure

• [PDB|1T90] [Pubmed|22782904]

Expression and Regulation

Operons

• genes: [gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]-[gene|0903CCBE053230998A9123F3269868E952C0F76B|iolB]-[gene|AAE60753464EAA32379521531FDCAF64534D5F40|iolC]-[gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]-[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]-[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]-[gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]-[gene|FD64E9D00BB03B510C7F7CA3935558A2195532A9|iolH]-[gene|2742FCE60AA87B2BE5A0B5C34F617E10DDE7F126|iolI]-[gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]
  description: [Pubmed|9226270]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9226270], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR]: repression, [Pubmed|9887260,9226270], in [regulon|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by inositol ([protein|search|IolR]) [Pubmed|9887260,9226270]

Biological materials

Mutant

• MGNA-B770 (iolA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1769 NBRP B. subtilis, Japan]
• BKE39760 ([gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA, downstream forward: _UP4_TAAACGACGAACAGCCGAAC
• BKK39760 ([gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA, downstream forward: _UP4_TAAACGACGAACAGCCGAAC

labs

• [SW|Yasutaro Fujita], University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

• 9226270,11566986,10666464,18310071,9887260,9887260,18310071,16332250,22900538