aroA

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase / chorismate mutase-isozyme 3

locus: BSU_29750

pI: 5.34

mw: 39.38 kDa

protein length: 358 aa

gene length: 1077 bp

function: biosynthesis of aromatic amino acids

product: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase /

essential: no

ec: 2.5.1.54

synonyms: aroG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.13|Biosynthesis/ acquisition of aromatic amino acids]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

Gene

Coordinates: 3,045,445 3,046,521

The protein

Catalyzed reaction/ biological activity

• D-erythrose 4-phosphate + H₂O + phosphoenolpyruvate --> 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate (according to UniProt)
• chorismate --> prephenate (according to UniProt)

Protein family

• class-I DAHP synthase family (single member, according to UniProt)

[SW|Domains]

• Chorismate mutase domain (aa 1-90) (according to UniProt)

Modification

• phosphorylation on Ser-2 [Pubmed|17218307]
• phosphorylation on Thr-4 [Pubmed|17726680]
• phosphorylated on Arg-45 and Arg-301 [Pubmed|22517742]
• Cys126 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|22938038]

Effectors of protein activity

• subject to feedback inhibition [Pubmed|19258532]

Structure

• [PDB|1VR6] (from ''Thermotoga maritima'', 51% identity, 68% similarity)
• [PDB|5GO2] (chorismate mutase-like domain, with citrate) [pubmed|28743924]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|75E331ABCDE1C60AAB4AC01229CED81B0AF50E1C|aroA]
  description: [Pubmed|9387221]

Biological materials

Mutant

• BKE29750 ([gene|75E331ABCDE1C60AAB4AC01229CED81B0AF50E1C|aroA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29750 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTTCATCCTTTCCC, downstream forward: _UP4_TAATTGAACAATCCAAAAGG
• BKK29750 ([gene|75E331ABCDE1C60AAB4AC01229CED81B0AF50E1C|aroA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTTCATCCTTTCCC, downstream forward: _UP4_TAATTGAACAATCCAAAAGG

References

• 22938038,9387221,19258532,17726680,17218307,22517742,15378759,28743924