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aroA
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aroA

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase / chorismate mutase-isozyme 3
Locus
BSU_29750
Isoelectric point
5.34
Molecular weight
39.38 kDa
Protein length
358 aa Sequence Blast
Gene length
1077 bp Sequence Blast
Function
biosynthesis of aromatic amino acids
Product
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase /
Essential
no
E.C.
2.5.1.54
Synonyms
aroG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aromatic amino acids
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

    • Gene

    Coordinates
    3,045,445 3,046,521

    The protein

    Catalyzed reaction/ biological activity

  • D-erythrose 4-phosphate + H2O + phosphoenolpyruvate --> 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate (according to UniProt)
  • chorismate --> prephenate (according to UniProt)

    • Protein family

  • class-I DAHP synthase family (single member, according to UniProt)

    • Domains

  • Chorismate mutase domain (aa 1-90) (according to UniProt)

    • Modification

  • phosphorylation on Ser-2 PubMed
  • phosphorylation on Thr-4 PubMed
  • phosphorylated on Arg-45 and Arg-301 PubMed
  • Cys126 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed

    • Effectors of protein activity

  • subject to feedback inhibition PubMed

    • Structure

  • 1VR6 (from Thermotoga maritima, 51% identity, 68% similarity)
  • 5GO2 (chorismate mutase-like domain, with citrate) PubMed

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    aroA
    Description
    PubMed
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    Biological materials

    Mutant

  • BKE29750 (aroA::erm trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_CATTTTTTTCATCCTTTCCC, downstream forward: _UP4_TAATTGAACAATCCAAAAGG
  • BKK29750 (aroA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTTCATCCTTTCCC, downstream forward: _UP4_TAATTGAACAATCCAAAAGG

    • References

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