ganP [2019-05-31 14:51:06]

galactotriose [SW|ABC transporter] (permease)

locus: BSU34150

pI: 6.09

mw: 46.82 kDa

protein length: 418 aa

gene length: 1257 bp

function: uptake of galactotriose

product: galactotriose [SW|ABC transporter] (permease)

essential: no

synonyms: yvfL

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.1|Uptake of carbon sources]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.24|Utilization of galactan]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,505,957 3,507,213

Phenotypes of a mutant

• a ''[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]'' mutant is impaired in the utilization of galactan [Pubmed|22893383]

The protein

Paralogous protein(s)

• [protein|EA2DD9892C1128ADCD6C8BC61248EBE45339E6D6|MdxF]

Structure

• [PDB|2R6G] (from E. coli, C-terminal domain, aa 165-412, 41% identity) [pubmed|18033289]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]
  description: [Pubmed|9287030]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|27501980], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1F787F70C87DEB221709579122D63C2322A84E56|GanR]: repression, [Pubmed|9287030], in [regulon|1F787F70C87DEB221709579122D63C2322A84E56|GanR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [pubmed|28617843], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by galactan (binding of galactobiose to [protein|1F787F70C87DEB221709579122D63C2322A84E56|GanR]) [Pubmed|28617843,27501980]
  • repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|28617843,27501980]

Biological materials

Mutant

• MGNA-A485 (yvfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/485 NBRP B. subtilis, Japan]
• BKE34150 ([gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCGTTTTCACCTCTCTTA, downstream forward: _UP4_TCTTTTAAAGAGGAGGCATA
• BKK34150 ([gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCGTTTTCACCTCTCTTA, downstream forward: _UP4_TCTTTTAAAGAGGAGGCATA

References

• 10092453,9287030,22893383,27501980,18033289