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rpsJ [2017-07-26 17:26:35]
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rpsJ [2017-07-26 17:26:35]

ribosomal protein
Locus
BSU01150
Isoelectric point
10.00
Molecular weight
11.00 kDa
Protein length
102 aa Sequence Blast
Gene length
306 bp Sequence Blast
Function
translation
Product
ribosomal protein S10 (BS13)
Essential
yes
Synonyms
tetA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins
  • Groups of genes → Essential genes
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue
  • Groups of genes → Universally conserved proteins

    • Gene

    Coordinates
    135,364 → 135,672

    Phenotypes of a mutant

  • essential PubMed

    • The protein

    Protein family

  • ribosomal protein S10P family (according to Swiss-Prot)

    • Modification

  • phosphorylated on Ser-32 during germination PubMed

    • Structure

  • 3J9W (the ribosome) PubMed

    • Localization

  • located near the aminoacyl-tRNA site of the 30S ribosomal subunit PubMed

    • Expression and Regulation

    Operons

    Genes
    rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-ybzG-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in SigA regulon

    • Regulatory mechanism

  • stringent response: negative regulation, in stringent response

    • Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed

    • Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed
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    Biological materials

    LacZ fusion

  • pGP605 (in pAC6), available in Jörg Stülke's lab
  • pGP2201 (in pAC7), available in Jörg Stülke's lab
  • pGP2439 (in pAC7), available in Jörg Stülke's lab
  • pGP2472 (in pAC5), available in Jörg Stülke's lab
  • pGP2473 (in pAC6), available in Jörg Stülke's lab

    • References

    Reviews

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    Original publications

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