rpsJ

ribosomal protein

Locus: BSU_01150

Isoelectric point: 10.00

Molecular weight: 11.00 kDa

Protein length: 102 aa

Gene length: 309 bp

Function: translation

Product: ribosomal protein S10 (BS13)

Essential: yes

Synonyms: tetA

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins
• Groups of genes → Essential genes
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue
• Groups of genes → Universally conserved proteins

Gene

Coordinates: 135,364 135,672

Phenotypes of a mutant

• essential PubMed

The protein

Protein family

• universal ribosomal protein uS10 family (single member, according to UniProt)

Modification

• phosphorylated on Ser-32 during germination PubMed

Structure

• 3J9W (the ribosome) PubMed
• 3R2C (the NusB-RpsJ complex from Aquifex aeolicus) PubMed

Localization

• located near the aminoacyl-tRNA site of the 30S ribosomal subunit PubMed

Expression and Regulation

Operons

• Genes: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-ybzG-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed

  Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed

Biological materials

LacZ fusion

• pGP605 (in pAC6), available in Jörg Stülke's lab
• pGP2201 (in pAC7), available in Jörg Stülke's lab
• pGP2439 (in pAC7), available in Jörg Stülke's lab
• pGP2472 (in pAC5), available in Jörg Stülke's lab
• pGP2473 (in pAC6), available in Jörg Stülke's lab
• pGP2828 (in pAC6), available in Jörg Stülke's lab, only hairpin 1
• pGP2829 (in pAC6), available in Jörg Stülke's lab, hairpins 1 and 2
• pGP2830 (in pAC6), available in Jörg Stülke's lab, all three hairpins

References

Reviews

Loading

Original publications

Loading