ascR [2019-09-03 09:26:47]

alkyl-sulphur catabolism regulator, transcriptional activator of the [gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|search|ytnM ]operon

locus: BSU_29400

pI: 6.23

mw: 35.08 kDa

protein length: 308 aa

gene length: 927 bp

function: regulation of sulphur metabolism

product: alkyl-sulphur catabolism regulator, transcriptional activator ([SW|LysR family])

essential: no

synonyms: ytlI

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.1|Conversion of S-methyl cysteine to cysteine]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 3,008,938 3,009,864

The protein

Protein family

• [SW|LysR family] (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|741156D495BE3857683C8A0390764EAD83845ABC|ascR]
  description: [Pubmed|9387221]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16109943], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16109943], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

  regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16109943]

Biological materials

Mutant

• MGNA-A169 (ytlI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/169 NBRP B. subtilis, Japan]
• BKE29400 ([gene|741156D495BE3857683C8A0390764EAD83845ABC|ascR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGCTCCCTCCTGTTT, downstream forward: _UP4_TAACTGAAAAAATAAAAACC
• BKK29400 ([gene|741156D495BE3857683C8A0390764EAD83845ABC|ascR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGCTCCCTCCTGTTT, downstream forward: _UP4_TAACTGAAAAAATAAAAACC

References

• 15937167,11390694,16109943,16513748,9387221,16109943,23944997