trmB

tRNA (m7G46) methyltransferase

locus: BSU_29900

pI: 6.35

mw: 24.36 kDa

protein length: 213 aa

gene length: 642 bp

function: tRNA modification

product: tRNA (m7G46) methyltransferase

essential: no

ec: 2.1.1.33

synonyms: ytmQ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 3,059,547 3,060,188

The protein

Catalyzed reaction/ biological activity

• guanosine⁴⁶ in tRNA + S-adenosyl-L-methionine --> N7-methylguanosine⁴⁶ in tRNA + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• [SW|Methyltransferase superfamily] (according to UniProt)
• [SW|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

[SW|Cofactors]

• S-adenosylmethionine [pubmed|16600901]

Structure

• [PDB|2FCA] [pubmed|16600901]

Expression and Regulation

Operons

• genes: [gene|73ED086EED7CECBE2FCADFBC964F1263CF825DB0|trmB]
  description: [Pubmed|9387221]

Biological materials

Mutant

• GP2649 ([gene|73ED086EED7CECBE2FCADFBC964F1263CF825DB0|trmB]::''cat''), available in [SW|Jörg Stülke]'s lab
• MGNA-A435 (ytmQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/435 NBRP B. subtilis, Japan]
• BKE29900 ([gene|73ED086EED7CECBE2FCADFBC964F1263CF825DB0|trmB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGATTGACACCTCTAT, downstream forward: _UP4_TAAAGACAGACTCTGACAGG
• BKK29900 ([gene|73ED086EED7CECBE2FCADFBC964F1263CF825DB0|trmB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGATTGACACCTCTAT, downstream forward: _UP4_TAAAGACAGACTCTGACAGG

References

• 16600901,9387221,27965289