SubtiBank SubtiBank
yhfR
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yhfR

phosphatase involved in isopentenol (isoprenoid) biosynthesis
Locus
BSU_10340
Isoelectric point
5.16
Molecular weight
21.85 kDa
Protein length
193 aa Sequence Blast
Gene length
582 bp Sequence Blast
Function
isoprenoid biosynthesis
Product
unknown
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of isoprenoids

    • Gene

    Coordinates
    1,108,736 1,109,317

    Phenotypes of a mutant

  • no detectable phenotype PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • phosphatase involved in isopentenol (isoprenoid) biosynthesis

    • Protein family

  • similar to 2,3-diphosphoglycerate-dependent phosphoglycerate mutases PubMed

    • Structure

  • 1H2F (enzyme of B. stearothermophilus) PubMed

    • Additional information

  • The gene is annotated in KEGG as an ortholog of phosphoglycerate mutase (PGM) EC 5.4.2.1. In MetaCyc the protein is marked as similar to phosphoglycerate mutase. No EC annotation is available in Swiss-Prot. Pearson et al. (PubMed) demonstrated that yhfR is non-essential for growth, sporulation, and spore germination. They also purified the gene, expressed it in B. subtilis but were not able to detect PGM activity in B. subtilis. PubMed

    • Expression and Regulation

    Operons

    Genes
    yhfR
    Description
    PubMed

    Regulatory mechanism

  • BirA: repression, in BirA regulon

    • Additional information

  • weakly expressed PubMed
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    Genes
    yhfU-yhfT-yhfS-yhfR
    Description
    PubMed

    Regulatory mechanism

  • BirA: repression, PubMed, in BirA regulon

    • Regulation

  • repressed in the presence of biotin (BirA) PubMed

    • Additional information

  • weakly expressed PubMed
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    Genes
    yhfT-yhfS-yhfR
    Description
    PubMed

    Additional information

  • weakly expressed PubMed
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    Biological materials

    Mutant

  • MGNA-A243 (yhfR::erm), available at the NBRP B. subtilis, Japan
  • BKE10340 (yhfR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAATCCCCTTCCTGAC, downstream forward: _UP4_TAAGAAAAAGACAGGCGTTT
  • BKK10340 (yhfR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAATCCCCTTCCTGAC, downstream forward: _UP4_TAAGAAAAAGACAGGCGTTT

    • References

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