yodB [2018-02-12T09:54:59.000Z]

MarR/DUF24 family transcription repressor of azoR1, catD-catE and yodC expression

Locus: BSU19540

Isoelectric point: 4.74

Molecular weight: 12.71 kDa

Protein length: 112 aa

Gene length: 336 bp

Function: regulation of quinone and diamide detoxification

Product: MarR/DUF24 family transcription repressor

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

Gene

Coordinates: 2,127,345 → 2,127,683

Phenotypes of a mutant

• increased resistance to catechol, quinones and diamide PubMed

The protein

Protein family

• MarR/DUF24 family

Paralogous protein(s)

• CatR, YdzF

Modification

• redox-controlled by intersubunit disulfide formation of Cys6 and Cys101' in response to quinones and diamide

Effectors of protein activity

• responds distinctly to diamide or methyl-p-benzoquinone PubMed

Structure

• 5HS7 (reduced form) PubMed
• 5HS8 (diamide-treated form) PubMed
• 5HS9 (quinone-bound form) PubMed

Additional information

• information on binding sites can be found in the PRODORIC2 database

Expression and Regulation

Operons

• Genes: yodB
  Description: PubMed

  Regulatory mechanism

  • YodB: repression, in YodB regulon

Biological materials

Mutant

• MGNA-B433 (yodB::erm), available at the NBRP B. subtilis, Japan
• BKE19540 (ΔyodB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGTCTTCATCCCTTCA, downstream forward: _UP4_TAAGGAAAGCGGCACCTGAA
• BKK19540 (ΔyodB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGTCTTCATCCCTTCA, downstream forward: _UP4_TAAGGAAAGCGGCACCTGAA

Labs working on this gene/protein

• Haike Antelmann,University of Greifswald, Germany

References

Reviews

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Original Publications

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