SubtiBank SubtiBank
yhxD [2018-02-13 15:39:05]
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yhxD [2018-02-13 15:39:05]

general stress protein, similar to alcohol dehydrogenase,
locus
BSU10430
pI
4.54
mw
31.76 kDa
protein length
299 aa Sequence Blast
gene length
897 bp Sequence Blast
function
survival of salt and ethanol stresses
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    1,117,734 → 1,118,633

    The protein

    Protein family

  • short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|EAEEA4DD9641919830A81185333A2610B964D37C|YhdF] (47%), [protein|3161519994609DA9360ED6E073E4A4C8C6210EFB|YdaD] (48,8%),[protein|6047F2493FCE3D2A9BDB1AD88E5926ECEED81036|YhxC] (45,5%), [protein|0DD474462720CAEE2AE17017BD7CA385238EBC6F|YxbG] (34,3%), [protein|CA4597C6253CCF7D7954686A30AF041808BDF8E5|Gdh] (33,5%), [protein|20A7DBA142F3BF8DCAD9732BD908CE8CC79AA993|BacC] (35,4%), [protein|439B468A13137000FB42E9389391CB4986FFED84|FabG] (31,5%), [protein|B6FF689E65906186F3576B378650D713DB84EDDA|YcdF] (31,1%), [protein|4DEFC2998464BF8327578C36A13A10DD277F991E|YkvO] (34,1%)

    • Structure

  • [PDB|3R3S] (from ''Salmonella Enterica'', 66% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|739B228743BC1FE9E6888E999BC0E4F615E36F9E|yhxD]
    description
    [Pubmed|8196543]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

    • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
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    Biological materials

    Mutant

  • MGNA-A719 (yhxD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/719 NBRP B. subtilis, Japan]
  • BKE10430 (Δ[gene|739B228743BC1FE9E6888E999BC0E4F615E36F9E|yhxD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGCAACGCTCCTTTTC, downstream forward: _UP4_TAGCTTTATGAAAGGAGCTG
  • BKK10430 (Δ[gene|739B228743BC1FE9E6888E999BC0E4F615E36F9E|yhxD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGCAACGCTCCTTTTC, downstream forward: _UP4_TAGCTTTATGAAAGGAGCTG

    • References

  • 12884008,8196543,15805528