cotA

laccase, bilirubin oxidase, spore coat protein (outer)

Locus: BSU_06300

Isoelectric point: 5.89

Molecular weight: 58.33 kDa

Protein length: 513 aa

Gene length: 1542 bp

Function: resistance of the spore

Product: laccase, bilirubin oxidase

Essential: no

E.C.: 1.3.3.5

Synonyms: pig

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class V
• Groups of genes → Efp-dependent proteins

Gene

Coordinates: 683,462 685,003

The protein

Protein family

• S.antibioticus phenoxazinone synthase (PhsA)

Structure

• 2BHF (reduced form)

Localization

• outer spore coat, more abundant at the mother cell-distal pole of the forespore, localization depends on CotE PubMed

Expression and Regulation

Operons

• Genes: cotA
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon

  Regulatory mechanism

  • GerE: repression, PubMed, in GerE regulon

  Regulation

  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

  Additional information

  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Mutant

• 1S101 ( cotA::cat), PubMed, available at BGSC
• 1S134 ( cotA::kan), PubMed, available at BGSC
• BKE06300 (cotA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCATCTGTCCTTATC, downstream forward: _UP4_TAACCCGACAAACTTGCCTC
• BKK06300 (cotA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCATCTGTCCTTATC, downstream forward: _UP4_TAACCCGACAAACTTGCCTC

References

Reviews

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Original publications

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