yflS

malate transporter

Locus: BSU_07570

Isoelectric point: 9.74

Molecular weight: 51.27 kDa

Protein length: 478 aa

Gene length: 1437 bp

Function: malate uptake

Product: malate transporter

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Transporter for organic acids
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
• Groups of genes → Membrane proteins

Gene

Coordinates: 829,382 830,818

RNA

Additional information

• the mRNA is stabilized by basepairing with the RoxS RNA PubMed
• non-protected mRNA is subject to degradation by by RNase J1 PubMed

The protein

Catalyzed reaction/ biological activity

• uptake of malate

Protein family

• SLC13A/DASS transporter (TC 2.A.47) family (single member, according to UniProt)

Localization

• cell membrane (according to UniProt)
• spore membrane PubMed

Expression and Regulation

Operons

• Genes: yflS
  Description: PubMed

  Regulatory mechanism

  • MalR: activation, in MalR regulon

  Regulation

  • induced by malate (MalR)

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the mRNA is stabilized by basepairing with the RoxS RNA PubMed
  • non-protected mRNA is subject to degradation by by RNase J1 PubMed

Biological materials

Mutant

• MGNA-C249 (yflS::erm), available at the NBRP B. subtilis, Japan
• GP1450 (cat), available in Jörg Stülke's lab
• BKE07570 (yflS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG
• BKK07570 (yflS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG

References

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