pbpF [2020-10-14 10:33:08]

class A penicillin-binding protein 2C

locus: BSU_10110

pI: 7.08

mw: 79.10 kDa

protein length: 714 aa

gene length: 2145 bp

function: bifunctional glucosyltransferase/ transpeptidase, synthesis of spore peptidoglycan

product: penicillin-binding protein 2C

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,083,851 1,085,995

Phenotypes of a mutant

• a strain lacking all four class A [SW|penicillin-binding proteins] ([gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|E50D4B30C2A0987A356C988AF8A4951C0CDF6C06|pbpD] [gene|73275060537497E6A9859856C9F040763E36316B|pbpF] [gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]) is severely inhibited for L-form switching in the presence of D-cycloserine [pubmed|29456081]

The protein

Catalyzed reaction/ biological activity

• synthesis of spore peptidoglycan (with [protein|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|PbpG]) [pubmed| 11567005]
• [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)]_(n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol = [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)]_(n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H⁺ (according to UniProt)
• Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

Protein family

• N-terminal part: [SW|glycosyltransferase 51 family] (according to UniProt)
• C-terminal part: [SW|transpeptidase family] (according to UniProt)

Paralogous protein(s)

• [protein|E50D4B30C2A0987A356C988AF8A4951C0CDF6C06|PbpD], [protein|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|PbpG], [protein|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PonA]

Structure

• [PDB|2OLU] (from Staphylococcus aureus, aa 40-610, 32% identity) [pubmed|17347437]

[SW|Localization]

• cell membrane, the major part is exposed to the outside (according to UniProt)
• prespore [Pubmed|15758244]
• during vegetative growth: septal, low flourescence at periphery [pubmed|14731276]

Expression and Regulation

Operons

• genes: [gene|73275060537497E6A9859856C9F040763E36316B|pbpF]
  description: [Pubmed|1459957]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8335642], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15758244], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed in mother cell and prespore [Pubmed|15758244]

Biological materials

Mutant

• BKE10110 ([gene|73275060537497E6A9859856C9F040763E36316B|pbpF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCTCGCCTTT, downstream forward: _UP4_TAATGGAATTCGGCGATTTT
• BKK10110 ([gene|73275060537497E6A9859856C9F040763E36316B|pbpF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCTCGCCTTT, downstream forward: _UP4_TAATGGAATTCGGCGATTTT

GFP fusion

• 2084 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|73275060537497E6A9859856C9F040763E36316B|pbpF]::pSG1491 (cat P_xyl-gfpa-[gene|73275060537497E6A9859856C9F040763E36316B|pbpF]1-335) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

References

• 8335642,8606187,15758244,1459957,14731276,17347437,29456081,11567005