folD

methylenetetrahydrofolate dehydrogenase (NADP)

Locus: BSU_24310

Isoelectric point: 5.60

Molecular weight: 30.53 kDa

Protein length: 283 aa

Gene length: 852 bp

Function: formylation of Met-tRNA(fMet)

Product: methylenetetrahydrofolate dehydrogenase (NADP)

Essential: no

E.C.: 1.5.1.5

Synonyms: yqiA

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

Gene

Coordinates: 2,528,404 2,529,255

Phenotypes of a mutant

• essential PubMed, non-essential according to PubMed
• inactivation of folD suppresses the synthetic lethality of the defA defB double mutant PubMed

The protein

Catalyzed reaction/ biological activity

• (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP⁺ --> 5,10-methenyltetrahydrofolate + NADPH (according to UniProt)
• 5,10-methenyltetrahydrofolate + H₂O --> (6S)-10-formyltetrahydrofolate + H⁺ (according to UniProt)

Protein family

• tetrahydrofolate dehydrogenase/cyclohydrolase family (single member, according to UniProt)

Structure

• 1B0A (from E. coli, 52% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: nusB-folD
  Description: PubMed

  Regulatory mechanism

  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon
  • stringent response: negative regulation, in stringent response

  Regulation

  • repressed in the presence of purine nucleotides (PurR) PubMed

Biological materials

Mutant

• BKE24310 (folD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCTTTCCTCCTATGA, downstream forward: _UP4_TAGATAATTGGAGATATTCA
• BKK24310 (folD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCTTTCCTCCTATGA, downstream forward: _UP4_TAGATAATTGGAGATATTCA

References

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