fnr

transcriptional regulator of anaerobic genes

locus: BSU_37310

pI: 5.99

mw: 27.07 kDa

protein length: 238 aa

gene length: 717 bp

function: regulation of anaerobiosis, fermentation and overflow metabolism

product: transcription regulator (Crp family)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.1|Regulators of electron transport]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 3,831,512 3,832,228

The protein

[SW|Domains]

• HTH crp-type domain (aa 145-219) (according to UniProt)

[SW|Cofactors]

• [4Fe-4S] cluster [pubmed|29292548]

Effectors of protein activity

• contains an iron-sulfur cluster

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000005 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]
  description: [Pubmed|8846791]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8846791], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|10972836], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|NsrR]: repression, [Pubmed|16885456], in [regulon|EC6697D5D945B7E5083AFED9218748763C443278|NsrR regulon]
  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: activation, [Pubmed|8846791,16428414], in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]

  regulation

  • ''[protein|search|fnr]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

• genes: [gene|F14481DAA196868ACC1DE9EC8CBE593A2CBB6BE4|narK]-[gene|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]
  description: [Pubmed|8846791]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8846791], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|NsrR]: repression, [Pubmed|16885456], in [regulon|EC6697D5D945B7E5083AFED9218748763C443278|NsrR regulon]
  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: activation, [Pubmed|8846791,16428414], in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]

  regulation

  • ''[protein|search|fnr]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

Biological materials

Mutant

• BKE37310 ([gene|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGCTCACCTCGAAGAA, downstream forward: _UP4_TGAAAAAAACAAGGACAGCG
• BKK37310 ([gene|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGCTCACCTCGAAGAA, downstream forward: _UP4_TGAAAAAAACAAGGACAGCG

References

Reviews

• 23046954,30225854

The [SW|Fnr regulon]

• 16428414

Other original publications

• 8682783,10972836,16885456,8846791,21068385,30225854