fnr

transcriptional regulator of anaerobic genes

Locus: BSU_37310

Isoelectric point: 5.99

Molecular weight: 27.07 kDa

Protein length: 238 aa

Gene length: 717 bp

Function: regulation of anaerobiosis, fermentation and overflow metabolism

Product: transcription regulator (Crp family)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Regulators of electron transport
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 3,831,512 3,832,228

The protein

Domains

• HTH crp-type domain (aa 145-219) (according to UniProt)

Cofactors

• [4Fe-4S] cluster PubMed

Effectors of protein activity

• contains an iron-sulfur cluster

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• information on binding sites can be found in the PRODORIC2 database

Expression and Regulation

Operons

• Genes: fnr
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • NsrR: repression, PubMed, in NsrR regulon
  • Fnr: activation, PubMed, in Fnr regulon

  Regulation

  • fnr: expressed under anaerobic conditions (ResD) PubMed

• Genes: narK-fnr
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • NsrR: repression, PubMed, in NsrR regulon
  • Fnr: activation, PubMed, in Fnr regulon

  Regulation

  • fnr: expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE37310 (fnr::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGCTCACCTCGAAGAA, downstream forward: _UP4_TGAAAAAAACAAGGACAGCG
• BKK37310 (fnr::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGCTCACCTCGAAGAA, downstream forward: _UP4_TGAAAAAAACAAGGACAGCG

References

Reviews

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The Fnr regulon

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Other original publications

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