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nupO [2017-8-11 16:0:3]
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nupO [2017-8-11 16:0:3]

[SW|ABC transporter ]for guanosine (ATP-binding protein)
locus
BSU31550
pI
7.50
mw
56.13 kDa
protein length
510 aa Sequence Blast
gene length
1530 bp Sequence Blast
function
uptake of guanosine
product
[SW|ABC transporter ]for guanosine
essential
no
synonyms
yufO
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.1|Uptake of carbon sources]
  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,241,085 → 3,242,617

    Phenotypes of a mutant

  • a ''[gene|71546D1508EF17A208332E162CAE2C299987A569|nupO] [gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]
  • defective in the the ability to support the growth of ''Synechococcus leopoliensis'' CCAP1405/1 on agar media [Pubmed|25875741]

    • The protein

    Protein family

  • [SW|ABC transporter] family (according to Swiss-Prot)

    • Structure

  • [PDB|5XJY] (human protein, 30% identity) [pubmed|28602350]

    • [SW|Localization]

  • associated to the membrane (via [protein|0BB235F189D30C9BE798560C31DA59FF0112CFD9|NupP]-[protein|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|NupQ]) [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]-[gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]-[gene|0BB235F189D30C9BE798560C31DA59FF0112CFD9|nupP]-[gene|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]
    description
    [Pubmed|21926227]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21926227], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455,21699902,21926227], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

    • regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455,21699902,21926227]
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    Biological materials

    Mutant

  • MGNA-B554 (yufO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1553 NBRP B. subtilis, Japan]

    • References

  • 10092453,21926227,12618455,25875741