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yvcK [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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yvcK [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
Locus
BSU34760
Isoelectric point
5.00
Molecular weight
34.00 kDa
Function
localization of ponA|PBP1
Product
unknown
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Categories containing this gene/protein

This gene is a member of the following regulons

Gene

Coordinates on the chromosome (coding sequence)
3,570,546 -> 3,571,499

Phenotypes of a mutant

  • unable to grow with gluconeogenic substrates as single carbon source PubMed
  • filamentous or L-shape-like aberrant morphologies (suppressed by Mg ) PubMed, this is supressed by overexpression of MreB or by deletion of ponA ([ponA|search|PBP1] ) PubMed
  • The protein

    Protein family

  • UPF0052 family (according to Swiss-Prot)
  • Domains

  • phosphorylated on Thr-304 by PrkC, dephosphorylated by PrpC PubMed
  • Structure

  • 2O2Z (the protein from B. halodurans, 63% identity, 86% similarity)
  • Localization

  • localized as a helical-like pattern PubMed
  • Interactions

    Expression and Regulation

    Operon

    Sigma factor

    Regulation

  • very weak stimuation of expression by citrate and succinate PubMed
  • Additional information

  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
  • Biological materials

    Expression vector

  • pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Labs working on this gene/protein

  • Anne Galinier, University of Marseille, France
  • References

    Reviews

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    Original publications

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