yvcK [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

essential for growth under gluconeogenetic conditions, required for correct localization of PBP1

Locus: BSU34760

Isoelectric point: 5.00

Molecular weight: 34.00 kDa

Function: localization of ponA|PBP1

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan
• Cellular processes → Cell envelope and cell division → Cell shape
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan
• Groups of genes → Efp-dependent proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

Categories containing this gene/protein

• carbon core metabolism, cell shape, phosphoproteins

This gene is a member of the following regulons

• Efp-dependent proteins

Gene

Coordinates on the chromosome (coding sequence): 3,570,546 -> 3,571,499

Phenotypes of a mutant

• unable to grow with gluconeogenic substrates as single carbon source PubMed
• filamentous or L-shape-like aberrant morphologies (suppressed by Mg ) PubMed, this is supressed by overexpression of MreB or by deletion of ponA ([ponA|search|PBP1] ) PubMed

The protein

Protein family

• UPF0052 family (according to Swiss-Prot)

Domains

• phosphorylated on Thr-304 by PrkC, dephosphorylated by PrpC PubMed

Structure

• 2O2Z (the protein from B. halodurans, 63% identity, 86% similarity)

Localization

• localized as a helical-like pattern PubMed

Interactions

• YvcK-PrkC PubMed
• YvcK-PrpC PubMed

Expression and Regulation

Operon

• yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

Sigma factor

• SigA PubMed

Regulation

• very weak stimuation of expression by citrate and succinate PubMed

Additional information

• translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Expression vector

• pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

Labs working on this gene/protein

• Anne Galinier, University of Marseille, France

References

Reviews

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Original publications

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