SubtiBank SubtiBank
yvcK [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yvcK [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
locus
BSU34760
pI
5.00
mw
34.00 kDa
function
localization of ponA|PBP1
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|cell shape], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|Efp-dependent proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,570,546 -> 3,571,499

    Phenotypes of a mutant

  • unable to grow with gluconeogenic substrates as single carbon source [Pubmed|16272399]
  • filamentous or L-shape-like aberrant morphologies (suppressed by Mg ) [Pubmed|16272399], this is supressed by overexpression of [protein|search|MreB] or by deletion of ''[protein|search|ponA]'' ([ponA|search|PBP1] ) [Pubmed|21320184]

    • The protein

    Protein family

  • UPF0052 family (according to Swiss-Prot)

    • [SW|Domains]

  • phosphorylated on Thr-304 by [protein|search|PrkC], dephosphorylated by [protein|search|PrpC] [Pubmed|25012659]

    • Structure

  • [PDB|2O2Z] (the protein from ''B. halodurans'', 63% identity, 86% similarity)

    • [SW|Localization]

  • localized as a helical-like pattern [Pubmed|25012659,21320184]

    • [SW|Interactions]

  • [protein|search|YvcK]-[protein|search|PrkC] [Pubmed|25012659]
  • [protein|search|YvcK]-[protein|search|PrpC] [Pubmed|25012659]

    • Expression and Regulation

    Operon

  • ''[protein|search|yvcI]-[protein|search|yvcJ]-[protein|search|yvcK]-[protein|search|yvcL]-[protein|search|crh]-[protein|search|yvcN]'' [Pubmed|9237995]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|16272399]

    • Regulation

  • very weak stimuation of expression by citrate and succinate [Pubmed|16272399]

    • Additional information

  • [protein|search|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

    • Biological materials

    Expression vector

  • pGP736 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] lab

    • Labs working on this gene/protein

  • [SW|Anne Galinier], University of Marseille, France

    • References

    Reviews

  • 21371139

    • Original publications

  • 9237995,16272399,21320184,25012659,25047842,27806131