ydaK

general stress protein, may act as c-di-GMP receptor, required for extracellular polysaccharide synthesis by YdaL-YdaM-YdaN

Locus: BSU_04280

Isoelectric point: 6.70

Molecular weight: 32.53 kDa

Protein length: 283 aa

Gene length: 852 bp

Function: unknown

Product: putative c-di-GMP receptor protein, general stress protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Targets of second messengers → Targets of c-di-GMP
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Groups of genes → Membrane proteins

Gene

Coordinates: 480,013 480,864

The protein

Domains

• four transmembrane helices at the N-terminus, they are essential for activation of EPS formation by YdaL, YdaM, and YdaN PubMed
• contains a C-terminal degenerated GGDEF domain (aa 162-283) PubMed

Cofactors

• binds c-di-GPM at high concentration (K(D) for c-di-GMP: 1.1 myM) PubMed

Localization

• cell membrane, forms clusters at the cell poles and septa (with YdaM and YdaN) PubMed

Expression and Regulation

Operons

• Genes: ydaJ-ydaK-ydaL-ydaM-ydaN
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

  Regulation

  • expressed during stress (SigB) PubMed

Biological materials

Mutant

• MGNA-C074 (ydaK::erm), available at the NBRP B. subtilis, Japan
• GP1592 (kan) available in Jörg Stülke's lab
• JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed
• BKE04280 (ydaK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAAATAAGCAAATAATTTTT, downstream forward: _UP4_ATGAATGAACTATAAAGGAA
• BKK04280 (ydaK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAAATAAGCAAATAATTTTT, downstream forward: _UP4_ATGAATGAACTATAAAGGAA

References

Reviews

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Original publications

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