yvrHb [2019-02-01 14:16:35]

two-component response regulator (OmpR family)

Locus: BSU33221

Isoelectric point: 5.24

Molecular weight: 27.00 kDa

Protein length: 237 aa

Gene length: 714 bp

Function: regulation cell surface maintenance

Product: two-component response regulator (OmpR family)

Essential: no

Synonyms: yvrH

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 3,408,353 3,409,066

Phenotypes of a mutant

• unusual autolysis and higher susceptibility to cell envelope antibiotics (bacitracin, aztreonam, cefepime, fosfomycin) PubMed

The protein

Catalyzed reaction/ biological activity

• transcription activator PubMed

Protein family

• OmpR family of two-component response regulators

Modification

• phosphorylation (Asp) by YvrG PubMed

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• 2OQR (from Mycobacterium tuberculosis, 37% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yvrHb-yvrG-yvrE
  Description: PubMed

Biological materials

Mutant

• MGNA-B050 (yvrH::erm), available at the NBRP B. subtilis, Japan
• BKE33221 (yvrHb::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTTATGTTCCTTTCTG, downstream forward: _UP4_CCAAATCCTGAAGGCAAGCG
• BKK33221 (yvrHb::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTTATGTTCCTTTCTG, downstream forward: _UP4_CCAAATCCTGAAGGCAAGCG

References

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