yclJ

two-component response regulator ([SW|OmpR family]), active under anaerobic conditions

locus: BSU_03750

pI: 4.58

mw: 26.36 kDa

protein length: 227 aa

gene length: 684 bp

function: unknown

product: two-component response regulator ([SW|OmpR family])

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 426,577 427,260

The protein

Protein family

• [SW|OmpR family] of two-component response regulators

Paralogous protein(s)

• [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR], [protein|6A37531896205A9B894C81AB0563C216C0B52CD7|YkoG], [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]

[SW|Domains]

• [SW|Response regulatory domain] (aa 2-115) (according to UniProt)

Modification

• phosphorylated by [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|YclK] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• [PDB|2OQR] (RegX3 from Mycobacterium tuberculosis, 42% identity) [pubmed|17942407]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]-[gene|52BB660B0CAB36C74F1D47963235846B45AF78AB|yclK]
  description: [Pubmed|15375128]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|15375128], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|15375128]

Biological materials

Mutant

• MGNA-C007 (yclJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2005 NBRP B. subtilis, Japan]
• BKE03750 ([gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCCTCCGGTTGTT, downstream forward: _UP4_ACTGTGTGGGGAGTAGGGTA
• BKK03750 ([gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCCTCCGGTTGTT, downstream forward: _UP4_ACTGTGTGGGGAGTAGGGTA

References

• 10094672,20512483,15375128,23504016,17942407