acoR

transcriptional activator of the acoA-acoB-acoC-acoL operon

Locus: BSU_08100

Isoelectric point: 7.88

Molecular weight: 66.98 kDa

Protein length: 605 aa

Gene length: 1818 bp

Function: regulation of acetoin utilization

Product: transcriptional activator (for SigL-dependent promoter)

Essential: no

Synonyms: yfjG,yzcB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of acetoin
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 883,758 885,575

The protein

Protein family

• Transcription factors activating transcription at SigL-dependent promoters

Domains

• Sigma-54 factor interaction domain (aa 295-520) (according to UniProt)

Structure

• 5EXX (from Pseudomonas aeruginosa, C-terminal doamin, corresponds to aa 292 ... 598, 43% identity) PubMed

Expression and Regulation

Operons

• Genes: acoR-sspH
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • repressed by glucose (3.7-fold) (CcpA) PubMed

Biological materials

Mutant

• MGNA-C352 (acoR::erm), available at the NBRP B. subtilis, Japan
• 1A912 ( acoR::kan), PubMed, available at BGSC and in Jörg Stülke's lab
• BKE08100 (acoR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCTTGATCGCTCCTC, downstream forward: _UP4_TAAAGCATACTTCCTTCAGG
• BKK08100 (acoR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCTTGATCGCTCCTC, downstream forward: _UP4_TAAAGCATACTTCCTTCAGG

Labs working on this gene/protein

• Michel Debarbouille, Pasteur Institute, Paris, France Homepage

References

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