acoR

transcriptional activator of the [gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]-[gene|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|acoB]-[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]-[gene|search|acoL ]operon

locus: BSU_08100

pI: 7.88

mw: 66.98 kDa

protein length: 605 aa

gene length: 1818 bp

function: regulation of acetoin utilization

product: transcriptional activator (for [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]-dependent promoter)

essential: no

synonyms: yfjG,yzcB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.2|Utilization of acetoin]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 883,758 885,575

The protein

Protein family

• [SW|Transcription factors activating transcription at SigL-dependent promoters]

[SW|Domains]

• Sigma-54 factor interaction domain (aa 295-520) (according to UniProt)

Structure

• [PDB|5EXX] (from Pseudomonas aeruginosa, C-terminal doamin, corresponds to aa 292 ... 598, 43% identity) [pubmed|26712005]

Expression and Regulation

Operons

• genes: [gene|706864AAF36684AD5E46F30E9EB76315AB412700|acoR]-[gene|4A4A533542E47DE8114B697FADE4CB581E5FB66E|sspH]
  description: [Pubmed|11274109]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22900538], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • repressed by glucose (3.7-fold) ([protein|search|CcpA]) [Pubmed|12850135]

Biological materials

Mutant

• MGNA-C352 (acoR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2350 NBRP B. subtilis, Japan]
• 1A912 ( ''acoR''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A912&Search=1A912 BGSC] and in [SW|Jörg Stülke]'s lab
• BKE08100 ([gene|706864AAF36684AD5E46F30E9EB76315AB412700|acoR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTTGATCGCTCCTC, downstream forward: _UP4_TAAAGCATACTTCCTTCAGG
• BKK08100 ([gene|706864AAF36684AD5E46F30E9EB76315AB412700|acoR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTTGATCGCTCCTC, downstream forward: _UP4_TAAAGCATACTTCCTTCAGG

labs

• [SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

References

• 11274109,10368162,22900538,12850135,21906631,26712005