pgcA [2019-03-13 12:15:10]

alpha-phosphoglucomutase, required for UDP-glucose synthesis, inhibits [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] ring assembly (indirect effect due to a defect in UDP-glucose synthesis)

locus: BSU09310

pI: 4.91

mw: 62.73 kDa

protein length: 581 aa

gene length: 1746 bp

function: interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate

product: alpha-phosphoglucomutase

essential: no

ec: 5.4.2.2

synonyms: yhxB,gtaC,gtaE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 1,006,774 1,008,519

Phenotypes of a mutant

• cells are bent and distended due to the lack of glucolipids [Pubmed|27684739]
• the inactivation of ''[gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]'' suppresses the poor and filamentous growth of the ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]'' double mutant [Pubmed|24097947]
• inactivation of ''[gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]'' reduces sporulation efficiency to 0.1% that of wild type cells; smaller and wider mother cells with small forespores [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate (according to Swiss-Prot)

Protein family

• phosphohexose mutase family [pubmed|15238632]

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]

Structure

• [PDB|2Z0F] (from Thermus thermophilus, 27% identity)

Additional information

• PgcA inhibits [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] ring assembly (indirect effect due to a defect in UDP-glucose synthesis)[Pubmed|17662947]

Expression and Regulation

Operons

• genes: [gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B476 (yhxB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1475 NBRP B. subtilis, Japan]
• GP1743: BSB1 ''pgcA::aphA3'', available in [SW|Jörg Stülke]'s lab
• BKE09310 ([gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGTCCTCCTATGTA, downstream forward: _UP4_TAATTCAAGTATGAGCTGGG
• BKK09310 ([gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGTCCTCCTATGTA, downstream forward: _UP4_TAATTCAAGTATGAGCTGGG

References

Reviews

• 20735481

Original publications

• 15175311,17726680,16493705,15640167,17662947,22396664,24097947,26735940,27684739,15238632