pgcA [Fri Feb 03 2017 13:41:30 GMT+0100 (CET)]

alpha-phosphoglucomutase, required for UDP-glucose synthesis, inhibits FtsZ ring assembly (indirect effect due to a defect in UDP-glucose synthesis)

locus: BSU09310

pI: 4.00

mw: 62.00 kDa

function: interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate

product: alpha-phosphoglucomutase

essential: no

ec: 5.4.2.2

synonyms: yhxB, gtaC, gtaE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

[SW|Categories] containing this gene/protein

• [SW|cell wall synthesis], [SW|lipid metabolism/ other], [SW|phosphoproteins], [SW|sporulation/ other]

Gene

Coordinates on the chromosome (coding sequence): 1,006,774 -> 1,008,519

Phenotypes of a mutant

• cells are bent and distended due to the lack of glucolipids [Pubmed|27684739]
• the inactivation of ''[protein|search|pgcA]'' suppresses the poor and filamentous growth of the ''[protein|search|yvcL] [protein|search|zapA]'' double mutant [Pubmed|24097947]
• inactivation of ''[SW|pgcA]'' reduces sporulation efficiency to 0.1% that of wild type cells; smaller and wider mother cells with small forespores [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate (according to Swiss-Prot)

Protein family

• phosphohexose mutase family (according to Swiss-Prot)

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]

Additional information

• PgcA inhibits [protein|search|FtsZ] ring assembly (indirect effect due to a defect in UDP-glucose synthesis)[Pubmed|17662947]

Biological materials

Mutant

• MGNA-B476 (yhxB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1475 NBRP B. subtilis, Japan]
• GP1743: ''pgcA::aphA3'', available in [SW|Jörg Stülke]' lab
• GP1743: BSB1 ''pgcA::aphA3'', available in [SW|Jörg Stülke]' lab

References

Reviews

• 20735481

Original publications

• 15175311,17726680,16493705,15640167,17662947,22396664,24097947,26735940,27684739