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pgcA [2019-10-08 09:50:27]
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pgcA [2019-10-08 09:50:27]

alpha-phosphoglucomutase, required for UDP-glucose synthesis, inhibits [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] ring assembly (indirect effect due to a defect in UDP-glucose synthesis), possesses a secondary phosphoglucosamine mutase activity
locus
BSU_09310
pI
4.91
mw
62.73 kDa
protein length
581 aa Sequence Blast
gene length
1746 bp Sequence Blast
function
interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate
product
alpha-phosphoglucomutase
essential
no
ec
5.4.2.2
synonyms
yhxB,gtaC,gtaE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • Gene

    Coordinates
    1,006,774 1,008,519

    Phenotypes of a mutant

  • cells are bent and distended due to the lack of glucolipids [Pubmed|27684739]
  • the inactivation of ''[gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]'' suppresses the poor and filamentous growth of the ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]'' double mutant [Pubmed|24097947]
  • inactivation of ''[gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]'' reduces sporulation efficiency to 0.1% that of wild type cells; smaller and wider mother cells with small forespores [Pubmed|26735940]
  • resistant to phage SPO1 [pubmed|30811056]
  • a [gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA] [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] double mutant is not viable, this can be suppressed by overexpression of [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|GlmM] [pubmed|31589605]

    • The protein

    Catalyzed reaction/ biological activity

  • α-D-glucose 1-phosphate --> α-D-glucose 6-phosphate (according to UniProt)
  • D-glucosamine 6-phosphate --> α-D-glucosamine 1-phosphate (secondary activity, this activity is increased by a G47S mutation) [pubmed|31589605]

    • Protein family

  • phosphohexose mutase family (with [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|GlmM], [pubmed|15238632])

    • Modification

  • phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]

    • Structure

  • [PDB|2Z0F] (from Thermus thermophilus, 27% identity)

    • Additional information

  • PgcA inhibits [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] ring assembly (indirect effect due to a defect in UDP-glucose synthesis)[Pubmed|17662947]
  • a PgcA (G47S) variant suppresses the growth defect of a glmR mutant on gluconeogenic carbon sources in the absence of glucose due to the increased phosphoglucosamine mutase activity [pubmed|31589605]

    • Expression and Regulation

    Operons

    genes
    [gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B476 (yhxB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1475 NBRP B. subtilis, Japan]
  • GP1743: BSB1 ''pgcA::aphA3'', available in [SW|Jörg Stülke]'s lab
  • BKE09310 ([gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGTCCTCCTATGTA, downstream forward: _UP4_TAATTCAAGTATGAGCTGGG
  • BKK09310 ([gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGTCCTCCTATGTA, downstream forward: _UP4_TAATTCAAGTATGAGCTGGG

    • References

    Reviews

  • 20735481

    • Original publications

  • 15175311,17726680,16493705,15640167,17662947,22396664,24097947,26735940,27684739,15238632,30811056,31589605