hemB [2019-08-08 10:13:40]

porphobilinogen synthase

locus: BSU_28130

pI: 4.98

mw: 36.05 kDa

protein length: 324 aa

gene length: 975 bp

function: heme biosynthesis

product: porphobilinogen synthase

essential: no

ec: 4.2.1.24

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.6|Biosynthesis of heme/ siroheme]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 2,874,202 2,875,176

The protein

Catalyzed reaction/ biological activity

• 2 5-aminolevulinate = porphobilinogen + 2 H₂O (according to Swiss-Prot)

Protein family

• ALAD family (single member, according to UniProt)

Modification

• phosphorylated on Arg-217 [Pubmed|22517742]

[SW|Cofactors]

• zinc

Structure

• [PDB|1W5Q] (from ''Pseudomonas aeruginosa'', 48% identity) [Pubmed|15644204]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• count: 2
  genes: [gene|B1413EB525000744BE8E429E760220A13BC4530D|hemA]-[gene|88616BA1E853E11BE0CB18F06287CA4C68143148|hemX]-[gene|D266E22E07B849E2AEB0EF232C49A2FE1EA4E02B|hemC]-[gene|33459D4389F85D60311FCE770BBA0ED9802BA342|hemD]-[gene|70263A55987F110209367CF45AD65A584AD3077D|hemB]-[gene|A54B2C86638D474F5F892003B42C535B5B39A42E|hemL]
  description: [Pubmed|1672867]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1672867], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR]: repression, [Pubmed|11532148], in [regulon|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR regulon]

  regulation

  • induced by hydrogen peroxide ([protein|search|PerR]) [Pubmed|11532148]

Biological materials

Mutant

• BKE28130 ([gene|70263A55987F110209367CF45AD65A584AD3077D|hemB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCGGTGTCTATTAAATGATT, downstream forward: _UP4_TAATTTTATTCAGTTGACAG
• BKK28130 ([gene|70263A55987F110209367CF45AD65A584AD3077D|hemB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCGGTGTCTATTAAATGATT, downstream forward: _UP4_TAATTTTATTCAGTTGACAG

References

Reviews

• 28123057

Original Publications

• 10217486,11532148,1672867,4214010,804803,12926268,20823524,22517742,15644204