hemB

porphobilinogen synthase

Locus: BSU_28130

Isoelectric point: 4.98

Molecular weight: 36.05 kDa

Protein length: 324 aa

Gene length: 975 bp

Function: heme biosynthesis

Product: porphobilinogen synthase

Essential: no

E.C.: 4.2.1.24

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of heme/ siroheme
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 2,874,202 2,875,176

The protein

Catalyzed reaction/ biological activity

• 2 5-aminolevulinate --> porphobilinogen + 2 H₂O (according to UniProt)

Protein family

• ALAD family (single member, according to UniProt)

Modification

• phosphorylated on Arg-217 PubMed

Cofactors

• zinc

Structure

• 1W5Q (from Pseudomonas aeruginosa, 48% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Count: 2
  Genes: hemA-hemX-hemC-hemD-hemB-hemL
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PerR: repression, PubMed, in PerR regulon

  Regulation

  • induced by hydrogen peroxide (PerR) PubMed

Biological materials

Mutant

• BKE28130 (hemB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCGGTGTCTATTAAATGATT, downstream forward: _UP4_TAATTTTATTCAGTTGACAG
• BKK28130 (hemB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCGGTGTCTATTAAATGATT, downstream forward: _UP4_TAATTTTATTCAGTTGACAG

References

Reviews

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Original Publications

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