RNA polymerase sigma factor SigD
function
regulation of flagella, motility, chemotaxis and autolysis
product
RNA polymerase sigma factor SigD
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming][SW|Categories] containing this gene/protein
[SW|transcription], [SW|sigma factors and their control], [SW|motility and chemotaxis]This gene is a member of the following [SW|regulons]
[SW|CodY regulon], [SW|DegU regulon], [SW|SigD regulon], [SW|Spo0A regulon]Gene
Coordinates on the chromosome (coding sequence)
1,716,493 -> 1,717,257
Phenotypes of a mutant
Inactivation of ''[protein|search|alsR]'', ''[protein|search|sigD]'' and ''[protein|search|sigW]'' increases competitive fitness of ''Bacillus subtilis'' under non-sporulating conditions [Pubmed|22344650]mucoid phenotype due to the overproduction of poly-gamma-glutamate (because of the loss of ''[protein|search|motA]-[protein|search|motB]'' expression) [Pubmed|24296669]not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]The protein
Protein family
sigma-70 factor family (according to Swiss-Prot)Effectors of protein activity
interaction with [protein|search|FlgM] inhibits SigD activity [Pubmed|10207036]Structure
[PDB|1RP3] ([SW|SigD]-[SW|FlgM] complex from '' '')[SW|Interactions]
[protein|search|SigD]-[protein|search|FlgM] [Pubmed|10207036], [protein|search|SigD]-([protein|search|RpoB]-[protein|search|RpoC])Expression and Regulation
Operon
part of the [[fla-che operon]]''[protein|search|flgB]-[protein|search|flgC]-[protein|search|fliE]-[protein|search|fliF]-[protein|search|fliG]-[protein|search|fliH]-[protein|search|fliI]-[protein|search|fliJ]-[protein|search|ylxF]-[protein|search|fliK]-[protein|search|flgD]-[protein|search|flgE]-[protein|search|fliL]-[protein|search|fliM]-[protein|search|fliY]-[protein|search|cheY]-[protein|search|fliZ]-[protein|search|fliP]-[protein|search|fliQ]-[protein|search|fliR]-[protein|search|flhB]-[protein|search|flhA]-[protein|search|flhF]-[protein|search|flhG]-[protein|search|cheB]-[protein|search|cheA]-[protein|search|cheW]-[protein|search|cheC]-[protein|search|cheD]-[protein|search|sigD]-[protein|search|swrB]'' [Pubmed|9657996,8157612,15175317]''[protein|search|ylxF]-[protein|search|fliK]-[protein|search|flgD]-[protein|search|flgE]-[protein|search|fliL]-[protein|search|fliM]-[protein|search|fliY]-[protein|search|cheY]-[protein|search|fliZ]-[protein|search|fliP]-[protein|search|fliQ]-[protein|search|fliR]-[protein|search|flhB]-[protein|search|flhA]-[protein|search|flhF]-[protein|search|flhG]-[protein|search|cheB]-[protein|search|cheA]-[protein|search|cheW]-[protein|search|cheC]-[protein|search|cheD]-[protein|search|sigD]-[protein|search|swrB]'' [Pubmed|20233303]''[protein|search|sigD]-[protein|search|swrB]'' [Pubmed|9335309][SW|Sigma factor]
''[protein|search|flgB]'': [protein|search|SigA] [Pubmed|9657996], [protein|search|SigD] [Pubmed|9657996]''[protein|search|ylxF]'': [protein|search|SigD] [Pubmed|20233303]''[protein|search|sigD]'': [protein|search|SigA] [Pubmed|9335309]Regulation
see [[fla-che operon]]Regulatory mechanism
see [[fla-che operon]]Biological materials
Mutant
1A716 ( ''sigD''::''cat''), [Pubmed|2832368], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A716&Search=1A716 BGSC]DS6420 (marker-less in NCIB3610) [Pubmed|22329926]two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] labFLAG-tag construct
GP948 (ermC, based on [SW|pGP1087]), available in the [SW|Stülke] labReferences
Reviews
The [SW|SigD regulon]
Original publications
26122431,22344650,22329926,21097624,22956758,21856853,8955328,7708689,9648743,8169223,9096206,15175317,2498284,16357223,3127378,26170408,9144176,18820022,7602586,2832368,8195064,11987133,14651647,9335309,9657996,8157612,10809682,8932324,20233303,17850253,10207036,16428420,24296669,25099370,24386445,26244495 
