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yxeB [2017-12-29 10:52:35]
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yxeB [2017-12-29 10:52:35]

hydroxamate siderophore ABC transporter (only ferrioxamine) (binding protein)
locus
BSU39610
pI
5.53
mw
35.10 kDa
protein length
319 aa Sequence Blast
gene length
957 bp Sequence Blast
function
siderophore uptake
product
hydroxamate siderophore ABC transporter
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    4,067,183 → 4,068,148

    Phenotypes of a mutant

  • no growth with the xenosiderophore ferrioxamine E as single source of iron [Pubmed|23220087]

    • The protein

    Protein family

  • bacterial solute-binding protein 8 family (according to Swiss-Prot)

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]
  • membrane, lipid modification as retention signal [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|yxeB]

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • repressed unless the cells enter an iron starvation ([protein|search|Fur]) [Pubmed|12354229]
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    Biological materials

    Mutant

  • MGNA-B706 (yxeB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1705 NBRP B. subtilis, Japan]
  • BKE39610 (Δ[gene|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|yxeB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGAACCCTCCCACTA, downstream forward: _UP4_TAAAAAAACGGCACAGTCAT
  • BKK39610 (Δ[gene|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|yxeB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGAACCCTCCCACTA, downstream forward: _UP4_TAAAAAAACGGCACAGTCAT

    • References

  • 10092453,16672620,12354229,18957862,18763711,23220087