yxeB

hydroxamate siderophore [SW|ABC transporter] (only ferrioxamine) (binding protein)

locus: BSU_39610

pI: 5.53

mw: 35.10 kDa

protein length: 321 aa

gene length: 966 bp

function: siderophore uptake

product: hydroxamate siderophore [SW|ABC transporter]

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 4,067,183 4,068,148

Phenotypes of a mutant

• no growth with the xenosiderophore ferrioxamine E as single source of iron [Pubmed|23220087]

The protein

Protein family

• [SW|Bacterial solute-binding protein 8 family] (according to UniProt)

Paralogous protein(s)

• [protein|84CC89907EF39770F5BFACA82A11B04CF76EC5AA|FhuD]

[SW|Domains]

• [SW|Fe/B12 periplasmic-binding domain] (aa 58-316) (according to UniProt)

Structure

• [PDB|4FNA] ([protein|84CC89907EF39770F5BFACA82A11B04CF76EC5AA|FhuD] from Staphylococcus aureus, 35% identity) [pubmed|24606332]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]
• membrane, lipid modification as retention signal [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|yxeB]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • repressed unless the cells enter an iron starvation ([protein|search|Fur]) [Pubmed|12354229]

Biological materials

Mutant

• MGNA-B706 (yxeB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1705 NBRP B. subtilis, Japan]
• BKE39610 ([gene|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|yxeB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGAACCCTCCCACTA, downstream forward: _UP4_TAAAAAAACGGCACAGTCAT
• BKK39610 ([gene|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|yxeB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGAACCCTCCCACTA, downstream forward: _UP4_TAAAAAAACGGCACAGTCAT

References

• 10092453,16672620,12354229,18957862,18763711,23220087,24606332