yxeB

hydroxamate siderophore ABC transporter (only ferrioxamine) (binding protein)

Locus: BSU_39610

Isoelectric point: 5.53

Molecular weight: 35.10 kDa

Protein length: 321 aa

Gene length: 966 bp

Function: siderophore uptake

Product: hydroxamate siderophore ABC transporter

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of iron/ siderophores
• Cellular processes → Homeostasis → Acquisition of iron → ABC transporters for the uptake of iron/ siderophores
• Metabolism → Additional metabolic pathways → Iron metabolism → ABC transporters for the uptake of iron/ siderophores
• Groups of genes → Secreted proteins

Gene

Coordinates: 4,067,183 4,068,148

Phenotypes of a mutant

• no growth with the xenosiderophore ferrioxamine E as single source of iron PubMed

The protein

Protein family

• Bacterial solute-binding protein 8 family (according to UniProt)

Paralogous protein(s)

• FhuD

Domains

• Fe/B12 periplasmic-binding domain (aa 58-316) (according to UniProt)

Structure

• 4FNA (FhuD from Staphylococcus aureus, 35% identity) PubMed

Localization

• extracellular (signal peptide) PubMed
• membrane, lipid modification as retention signal PubMed

Expression and Regulation

Operons

• Genes: yxeB

  Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • repressed unless the cells enter an iron starvation (Fur) PubMed

Biological materials

Mutant

• MGNA-B706 (yxeB::erm), available at the NBRP B. subtilis, Japan
• BKE39610 (yxeB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAAGAACCCTCCCACTA, downstream forward: _UP4_TAAAAAAACGGCACAGTCAT
• BKK39610 (yxeB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAAGAACCCTCCCACTA, downstream forward: _UP4_TAAAAAAACGGCACAGTCAT

References

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