pyrR

transcriptional antiterminator of the pyr operon

Locus: BSU_15470

Isoelectric point: 4.99

Molecular weight: 20.12 kDa

Protein length: 181 aa

Gene length: 546 bp

Function: regulation of pyrimidine biosynthesis

Product: transcriptional antiterminator with minor uracil phosphoribosyltransferase activity

Essential: no

E.C.: 2.4.2.9

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of pyrimidine nucleotides
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Information processing → Regulation of gene expression → RNA binding regulators

Gene

Coordinates: 1,618,304 1,618,849

The protein

Catalyzed reaction/ biological activity

• diphosphate + UMP --> 5-phospho-α-D-ribose 1-diphosphate + uracil (according to UniProt)

Protein family

• Purine/pyrimidine phosphoribosyltransferase family (according to UniProt)

Effectors of protein activity

• UMP and UTP stimulate the RNA-binding activity of PyrR, resulting of transcription termination of the pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE operon PubMed

Structure

• 1A3C (dimeric form)

Additional information

• information on binding sites can be found in the PRODORIC2 database

Expression and Regulation

Operons

• Genes: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PyrR: termination/ antitermination, via RNA switch, in PyrR regulon

  Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed

• Genes: pyrR
  Description: PubMed

• Genes: lspA-ylyB-pyrR
  Description: PubMed

Biological materials

Mutant

• BKE15470 (pyrR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTGTGACACCTCACAG, downstream forward: _UP4_GCCATTTATGAAAACGAATA
• BKK15470 (pyrR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTGTGACACCTCACAG, downstream forward: _UP4_GCCATTTATGAAAACGAATA

References

Reviews

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Original publications

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