dapG

aspartokinase I (alpha and beta subunits)

locus: BSU_16760

pI: 5.71

mw: 42.82 kDa

protein length: 404 aa

gene length: 1215 bp

function: biosynthesis of lysine and peptidoglycan

product: aspartokinase I (alpha and beta subunits)

essential: yes

ec: 2.7.2.4

synonyms: lssD

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 1,747,123 1,748,337

Phenotypes of a mutant

• essential [Pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• ATP + L-aspartate --> 4-phospho-L-aspartate + ADP (according to UniProt)

Protein family

• aspartokinase family (with [protein|F7B947938FB40E54D89A719E9610C5A4AA400674|LysC] and [protein|548E92526BC00999031D6EDE43FE061CE0448AFC|ThrD], according to UniProt)

Paralogous protein(s)

• [protein|F7B947938FB40E54D89A719E9610C5A4AA400674|LysC]

[SW|Domains]

• [SW|ACT domain] (aa 344-404) (according to UniProt)

Effectors of protein activity

• inhibited by diaminopimelic acid [Pubmed|2152900]

Structure

• [PDB|1SFT] (from ''Geobacillus stearothermophilus'', 57% identity) [Pubmed|9063881]

Expression and Regulation

Operons

• genes: [gene|564A2F43F8AABE97FC8F0B1B3382A9F31F02FA18|asd]-[gene|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG]-[gene|00B4CA4E25858A56F9D4E0ADEE2643F203FEC613|dapA]
  description: [Pubmed|8098035]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,8098035], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8098035], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

• genes: [gene|3DA80060554B78E9DC2189CECB78680095B08B32|spoVFA]-[gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]-[gene|564A2F43F8AABE97FC8F0B1B3382A9F31F02FA18|asd]-[gene|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG]-[gene|00B4CA4E25858A56F9D4E0ADEE2643F203FEC613|dapA]
  description: [Pubmed|8098035]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,8098035], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,8098035]

References

Reviews

• 19946135,22079167

Original publications

• 8345520,2153658,15699190,8098035,2152900,9063881,28189581