yhaM [2019-08-30 17:43:16]

RNase, 3 5 exoribonuclease

locus: BSU_09930

pI: 5.85

mw: 35.53 kDa

protein length: 314 aa

gene length: 945 bp

function: RNA degradation

product: RNase YhaM

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.1|Exoribonucleases]

Gene

Coordinates: 1,069,042 1,069,986

The protein

Catalyzed reaction/ biological activity

• 3'-5' exoribonuclease

Protein family

• YhaM family (single member, according to UniProt)

[SW|Domains]

• [SW|HD domain] (aa 156 ... 297) (according to the Interpro database)

[SW|Localization]

• cytoplasm (Homogeneous) [Pubmed|16479537]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|3E27C881BB3DE3FD55B7AD21B8771A859D80786F|yhaO]-[gene|709C4D0AE9C78C9A367F37B9C3B37D2516850D7C|sbcE]-[gene|6D585EB5E56A1E5C1C31680D960043BA08C817E2|yhaM]
  description: [Pubmed|16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [Pubmed|16267290]

Biological materials

Mutant

• MGNA-B499 (yhaM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1498 NBRP B. subtilis, Japan]
• BKE09930 ([gene|6D585EB5E56A1E5C1C31680D960043BA08C817E2|yhaM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTATTAAAGCTCCCTC, downstream forward: _UP4_TAACTTAAAAAGGACTGATG
• BKK09930 ([gene|6D585EB5E56A1E5C1C31680D960043BA08C817E2|yhaM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTATTAAAGCTCCCTC, downstream forward: _UP4_TAACTTAAAAAGGACTGATG

labs

• [SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]

References

• 19732150,12399495,19553197,16267290,16479537,17981983,15805522,19880604