ppiB

peptidyl-prolyl isomerase

locus: BSU_23360

pI: 5.47

mw: 15.11 kDa

protein length: 143 aa

gene length: 432 bp

function: protein folding

product: peptidyl-prolyl isomerase

essential: no

ec: 5.2.1.8

synonyms: cypBS

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.2|Chaperones/ protein folding]

Gene

Coordinates: 2,435,360 2,435,791

The protein

Catalyzed reaction/ biological activity

• [protein]-peptidylproline (ω=180) --> [protein]-peptidylproline (ω=0) (according to UniProt)

Protein family

• cyclophilin-type PPIase family (single member, according to UniProt)

[SW|Domains]

• PPIase cyclophilin-type domain (aa 1-143) (according to UniProt)

Structure

• [PDB|2MVZ] (from Geobacillus kaustophilus, 75% identity) [pubmed|25923019]

Expression and Regulation

Operons

• genes: [gene|6D38B333DE44EB903E151004BA30F3361CC1BE7C|ppiB]
  description: [Pubmed|8022278]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8022278], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• BKE23360 ([gene|6D38B333DE44EB903E151004BA30F3361CC1BE7C|ppiB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGTATGCCTACTTTCT, downstream forward: _UP4_TAATCGGCAGGCAAAAGGCT
• BKK23360 ([gene|6D38B333DE44EB903E151004BA30F3361CC1BE7C|ppiB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGTATGCCTACTTTCT, downstream forward: _UP4_TAATCGGCAGGCAAAAGGCT

References

• 9748346,1516692,9297832,8639516,8022278,25923019