nagR [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

transcriptional regulator (GntR family)

locus: BSU35030

pI: 6.00

mw: 27.00 kDa

function: regulator of the nagA-nagB-nagR operon

product: transcriptional regulator (GntR family)

essential: no

synonyms: yvoA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.17|Utilization of amino sugars]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.3|Utilization of amino sugars]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources], [SW|transcription factors and their control]

This gene is a member of the following [SW|regulons]

• [SW|NagR regulon]

Gene

Coordinates on the chromosome (coding sequence): 3,597,289 -> 3,598,020

The protein

Catalyzed reaction/ biological activity

• transcription repression of ''[protein|search|nagP]'' and of the putative ''[protein|search|nagA]-[protein|search|nagB]-[protein|search|nagR]'' operon in the absence of N-acetylglycosamine [Pubmed|24673833,20047956,21602348], weak repression of ''[protein|search|yflG]'' [Pubmed|16207374]

Protein family

• [SW|GntR family] of transcription factors

Kinetic information

• K(D) for N-acetylglucosamine-6-phosphate: 1 mM [Pubmed|20047956]

Effectors of protein activity

• N-acetylglucosamine-6-phosphate and glucosamine-6-phosphate act as the molecular inducers [Pubmed|25564531,21602348,20047956]

Structure

• [PDB|2WV0] [Pubmed|20047956]
• [PDB|4U0V] (complex with glucosamine-6-phosphate) [Pubmed|25564531]
• [PDB|4U0W] (complex with N-acetylglucosamine-6-phosphate) [Pubmed|25564531]
• [PDB|4WWC] (complex with palindromic operator DNA) [Pubmed|25564531]

Expression and Regulation

Operon

• ''[protein|search|nagA]-[protein|search|nagB]-[protein|search|nagR]'' [Pubmed|21602348]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|23667565]

Regulation

• induced in the presence of N-acetylglucosamine ([protein|search|NagR]) [Pubmed|24673833,21602348]

Regulatory mechanism

• [protein|search|NagR]: transcription repression [Pubmed|24673833,21602348]

References

Reviews

• 26159076

Original publications

• 19342794,20047956,16207374,21602348,23667565,24673833,25564531