glnM

glutamine [SW|ABC transporter] (membrane protein)

locus: BSU_27450

pI: 8.42

mw: 23.55 kDa

protein length: 216 aa

gene length: 651 bp

function: glutamine uptake

product: glutamine [SW|ABC transporter] (membrane protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,803,990 2,804,640

The protein

Protein family

• [SW|Binding-protein-dependent transport system permease family] (according to UniProt)

Paralogous protein(s)

• [protein|CD32011A08697B2C121996A02F1BD7BE4F932778|GlnP]

[SW|Domains]

• [SW|ABC transmembrane type-1 domain] (aa 17-205) (according to UniProt)

Structure

• [PDB|4YMS] (complex with [protein|CD32011A08697B2C121996A02F1BD7BE4F932778|GlnP]; from ''Thermoanaerobacter tengcongensis '', 40% identity) [Pubmed|25848002]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|468BEA9EAEA589D683DB79B2C689A5C6A22AB67F|glnQ]-[gene|BAB70547672C855AEB3A4D4624044340CF582848|glnH]-[gene|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|glnM]-[gene|CD32011A08697B2C121996A02F1BD7BE4F932778|glnP]
  description: [Pubmed|12823818]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • expressed during sporulation ([protein|search|SigE]) [Pubmed|15699190]

Biological materials

Mutant

• BKE27450 ([gene|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|glnM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGCCTATTCACTCCTT, downstream forward: _UP4_AGCTATTGATAAGGAGTGAG
• BKK27450 ([gene|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|glnM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGCCTATTCACTCCTT, downstream forward: _UP4_AGCTATTGATAAGGAGTGAG

References

• 10092453,25755103,12823818,15699190,25848002,20023025