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ylbF [2020-03-12 12:29:28]
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ylbF [2020-03-12 12:29:28]

subunit of the regulatory iron-sulfur containing [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex, required for [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] dependent maturation of polycistronic mRNAs, antagonist of biofilm repression by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR], control of the [SW|phosphorelay]
locus
BSU_14990
pI
5.01
mw
16.77 kDa
protein length
149 aa Sequence Blast
gene length
450 bp Sequence Blast
function
control of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] activity, see [SW|Targets of the Y complex]
product
subunit of the regulatory iron-sulfur containing [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex
essential
no
synonyms
ricF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.5|Effectors of RNA degradation]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.6|Other protein controlling the activity of the phosphorelay]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.6|Other protein controlling the activity of the phosphorelay]

    • Gene

    Coordinates
    1,568,420 1,568,869

    Phenotypes of a mutant

  • strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]
  • defective in [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y]-mediated maturation of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]

    • The protein

    Catalyzed reaction/ biological activity

  • the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex acts as specificity factor for [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y]-dependent processing of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]
  • the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex stimulates [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A] phosphorylation in the [category|SW 4.2.2|phosphorelay] [pubmed|27501195]

    • [SW|Cofactors]

  • one 4Fe-4S cluster (contributes to the co-ordination of the cluster (with [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA] and [protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT])) [pubmed|31530674]

    • Structure

  • [PDB|6PRK] (the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF] complex) [pubmed|31530674]

    • [SW|Localization]

  • cytoplasm [pubmed|27501195]

    • Expression and Regulation

    Operons

    genes
    [gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]-[gene|FBD8F0EB241A35747E6398C9AA30D71D6D17CE99|ylbG]-[gene|65FA4B3738C2A5995F019099743C782ADD15C338|ylbH]-[gene|6DAAEF20B68139A732FDCDABD0C420A0C928D373|ylbI]
    description
    [Pubmed|22383849]
    view in new tab

    additional information

  • 7,000/ 340 molecules per cell during growth in LB/ minimal medium [pubmed|31530674]

    • Biological materials

    Mutant

  • BKE14990 ([gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14990 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG
  • BKK14990 ([gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

    • References

    Reviews

  • 32061882

    • Original publications

  • 10712692,15661000,23490197,15175311,26434553,28295778,27501195,29794222,31530674