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ylbF [2020-02-19 15:41:14]
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ylbF [2020-02-19 15:41:14]

subunit of the regulatory iron-sulfur containing YmcA-YlbF-YaaT complex, required for RNase Y dependent maturation of polycistronic mRNAs, antagonist of biofilm repression by SinR, control of the phosphorelay
Locus
BSU_14990
Isoelectric point
5.01
Molecular weight
16.77 kDa
Protein length
149 aa Sequence Blast
Gene length
450 bp Sequence Blast
Function
control of RNase Y activity, see Targets of the Y complex
Product
subunit of the regulatory iron-sulfur containing YmcA-YlbF-YaaT complex
Essential
no
Synonyms
ricF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → RNases → Effectors of RNA degradation
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)
  • Information processing → Regulation of gene expression → phosphorelay → Other protein controlling the activity of the phosphorelay
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for biofilm formation
  • Lifestyles → Sporulation → phosphorelay → Other protein controlling the activity of the phosphorelay

    • Gene

    Coordinates
    1,568,420 1,568,869

    Phenotypes of a mutant

  • strongly reduced genetic competence, strongly reduced sporulation PubMed
  • defective in RNase Y-mediated maturation of polycistronic mRNAs, see Targets of the Y complex PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • the YmcA-YlbF-YaaT complex acts as specificity factor for RNase Y-dependent processing of polycistronic mRNAs, see Targets of the Y complex PubMed
  • the YmcA-YlbF-YaaT complex stimulates Spo0A phosphorylation in the phosphorelay PubMed

    • Cofactors

  • one 4Fe-4S cluster (contributes to the co-ordination of the cluster (with YmcA and YaaT)) PubMed

    • Structure

  • 6PRK (the YmcA-YlbF complex) PubMed

    • Localization

  • cytoplasm PubMed

    • Expression and Regulation

    Operons

    Genes
    ylbF
    Description
    PubMed
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    Genes
    ylbF-ylbG-ylbH-ylbI
    Description
    PubMed
    view in new tab

    Additional information

  • 7,000/ 340 molecules per cell during growth in LB/ minimal medium PubMed

    • Biological materials

    Mutant

  • BKE14990 (ylbF::erm trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG
  • BKK14990 (ylbF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

    • References

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