ytvA [2019-11-11 10:25:50]

blue light sensor, positive regulation of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] activity under conditions of blue light

locus: BSU_30340

pI: 4.69

mw: 29.04 kDa

protein length: 261 aa

gene length: 786 bp

function: control of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] activity

product: blue light receptor, flavoprotein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]

Gene

Coordinates: 3,106,210 3,106,995

The protein

Catalyzed reaction/ biological activity

• mediates light-dependent stimulation of [protein|ADC52A22950736A0435AEEEC43F7407878786A81|RsbT] kinase activity [Pubmed|23416074]

Paralogous protein(s)

• [protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA], [protein|979D7A45EAD97C99015029400A85795061BAA367|RsbRD], [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|RsbRB], [protein|5EEEDE60CE9E5CDAEBAB0E03AABDF1DF62F1F002|RsbRC], [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|RsbR], [protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA]

[SW|Domains]

• [SW|PAS domain] (aa 12-87) (according to UniProt)
• [SW|PAC domain] (aa 88-138) (according to UniProt)
• [SW|STAS domain] (aa 147-258) (according to UniProt)

[SW|Cofactors]

• FMN

Effectors of protein activity

• [protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA]-dependent light activation of the [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] stress response is positively and negatively regulated by [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|RsbR] and [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|RsbRB], respectively [Pubmed|22287516]

Structure

• [PDB|2PR5] (dark), [PDB|2PR6] (light), [PDB|2MWG]

Expression and Regulation

Operons

• genes: [gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|16923906], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

Biological materials

Mutant

• MGNA-A136 (ytvA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/136 NBRP B. subtilis, Japan]
• 1A965 ( ''ytvA''::''erm''), [Pubmed|11157946], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A965&Search=1A965 BGSC]
• BKE30340 ([gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAATCCCCCTTAGGC, downstream forward: _UP4_TAAAAAGATCCCGCTCACCC
• BKK30340 ([gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAATCCCCCTTAGGC, downstream forward: _UP4_TAAAAAGATCCCGCTCACCC

Expression vectors

• pGP2685, expression of His-[protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA] in ''E. coli'' (based on [SW|pGP570]), available in [SW|Jörg Stülke]'s lab

labs

• [SW|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]

References

Reviews

• 18400553,21822294,26189730,28271471

Original publications

• 27203230,19581299,16923906,17575448,17764689,17443319,17200735,16923909,16797012,16022561,15339223,11964249,11157946,19891470,20062844,19783626,20800068,22247770,23047813,23416074,23456923,23817467,21259411,21388385,21410163,21851109,22287516,23861663,23828427,23900620,23940057,24171435,24278408,25211155,26402155