blue light sensor, positive regulation of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] activity under conditions of blue light
function
control of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] activity
product
blue light receptor, flavoprotein
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]Gene
Coordinates
3,106,210 3,106,995
The protein
Catalyzed reaction/ biological activity
mediates light-dependent stimulation of [protein|ADC52A22950736A0435AEEEC43F7407878786A81|RsbT] kinase activity [Pubmed|23416074]Paralogous protein(s)
[protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA], [protein|979D7A45EAD97C99015029400A85795061BAA367|RsbRD], [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|RsbRB], [protein|5EEEDE60CE9E5CDAEBAB0E03AABDF1DF62F1F002|RsbRC], [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|RsbR], [protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA] [SW|Domains]
[SW|PAS domain] (aa 12-87) (according to UniProt)[SW|PAC domain] (aa 88-138) (according to UniProt)[SW|STAS domain] (aa 147-258) (according to UniProt)[SW|Cofactors]
FMNEffectors of protein activity
[protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA]-dependent light activation of the [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] stress response is positively and negatively regulated by [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|RsbR] and [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|RsbRB], respectively [Pubmed|22287516]Structure
[PDB|2PR5] (dark), [PDB|2PR6] (light), [PDB|2MWG]Expression and Regulation
Operons
genes
[gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]
description
[pubmed|22383849]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|16923906], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]view in new tabBiological materials
Mutant
MGNA-A136 (ytvA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/136 NBRP B. subtilis, Japan]1A965 ( ''ytvA''::''erm''), [Pubmed|11157946], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A965&Search=1A965 BGSC]BKE30340 ([gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAATCCCCCTTAGGC, downstream forward: _UP4_TAAAAAGATCCCGCTCACCCBKK30340 ([gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAATCCCCCTTAGGC, downstream forward: _UP4_TAAAAAGATCCCGCTCACCCExpression vectors
pGP2685, expression of His-[protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA] in ''E. coli'' (based on [SW|pGP570]), available in [SW|Jörg Stülke]'s lablabs
[SW|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]References
Reviews
18400553,21822294,26189730,28271471 Original publications
27203230,19581299,16923906,17575448,17764689,17443319,17200735,16923909,16797012,16022561,15339223,11964249,11157946,19891470,20062844,19783626,20800068,22247770,23047813,23416074,23456923,23817467,21259411,21388385,21410163,21851109,22287516,23861663,23828427,23900620,23940057,24171435,24278408,25211155,26402155,31707658,32579655