acpK

acyl carrier protein

Locus: BSU_17130

Isoelectric point: 4.11

Molecular weight: 9.11 kDa

Protein length: 82 aa

Gene length: 249 bp

Function: polyketide biosynthesis

Product: acyl carrier protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds

Gene

Coordinates: 1,788,469 1,788,717

The protein

Domains

• Carrier domain (aa 4-79) (according to UniProt)

Structure

• 5KP7 (53% identity) PubMed

Expression and Regulation

Operons

• Genes: pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • CodY: activation, PubMed, in CodY regulon

  Regulation

  • expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed

  Additional information

  • this is a very large operon comprising about 75 kb

• Genes: pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
  Description: PubMed

Biological materials

Mutant

• BKE17130 (acpK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATGCGCTCTCTCCTTC, downstream forward: _UP4_ATCGGTGAATTAGCTGAGGT
• BKK17130 (acpK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATGCGCTCTCTCCTTC, downstream forward: _UP4_ATCGGTGAATTAGCTGAGGT

References

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