cah

cephalosporin C deacetylase

locus: BSU_03180

pI: 5.42

mw: 35.65 kDa

protein length: 318 aa

gene length: 957 bp

function: resistance to cephalosporin C

product: cephalosporin C deacetylase)

essential: no

ec: 3.1.1.41

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]

Gene

Coordinates: 342,538 343,494

The protein

Catalyzed reaction/ biological activity

• cephalosporin C + H₂O --> acetate + deacetylcephalosporin C + H⁺ (according to UniProt)

Protein family

• carbohydrate esterase 7 family (single member, according to UniProt)

Structure

• [PDB|1ODS] [pubmed|12842474]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|6BCE78F623A40B519211E79AFA1AD898318BD817|cah]
  description: [Pubmed|7793942]

• genes: [gene|D28190620D5B834DD7F42822B835C6FE932FD8A5|ycgK]-[gene|6BCE78F623A40B519211E79AFA1AD898318BD817|cah]-[gene|7306A55192AEB4032FD17A1BAAFF1127D511D70E|ycgL]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE03180 ([gene|6BCE78F623A40B519211E79AFA1AD898318BD817|cah]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGATTATTCCCCTT, downstream forward: _UP4_TGATAAATGTGAAAAGCCGC
• BKK03180 ([gene|6BCE78F623A40B519211E79AFA1AD898318BD817|cah]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGATTATTCCCCTT, downstream forward: _UP4_TGATAAATGTGAAAAGCCGC

References

• 12842474,7793942,15069587,16232499,12842474