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cotR
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cotR

outer spore coat protein, lipolytic enzyme
Locus
BSU_34530
Isoelectric point
10.20
Molecular weight
35.20 kDa
Protein length
320 aa Sequence Blast
Gene length
963 bp Sequence Blast
Function
hydrolysis of lysophospholipids
Product
outer spore coat lipolytic enzyme
Essential
no
Synonyms
yvdO

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,544,642 3,545,604

The protein

Catalyzed reaction/ biological activity

  • release of myristic acid from 1-myristoly-2-lyso-sn-glycero-3-phosphocholine PubMed
  • Domains

  • PNPLA domain (aa 7-182) (according to UniProt)
  • Localization

  • outer spore coat PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon
  • Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed
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    Biological materials

    Mutant

  • MGNA-B621 (yvdO::erm), available at the NBRP B. subtilis, Japan
  • BKE34530 (cotR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAGCTGATGTCTCCTTTC, downstream forward: _UP4_TAATTATTTATTTTAAATCA
  • BKK34530 (cotR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAGCTGATGTCTCCTTTC, downstream forward: _UP4_TAATTATTTATTTTAAATCA
  • References

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