yolJ [2018-11-19 19:04:52]

phage-derived sublancin S-glycosyltransferase

locus: BSU21450

pI: 5.33

mw: 49.60 kDa

protein length: 422 aa

gene length: 1266 bp

function: biosynthesis of the antimicrobial peptide sublancin

product: sublancin S-glycosyltransferase

essential: no

synonyms: yolJ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.8|Protein modification/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]

Gene

Coordinates: 2,265,668 → 2,266,936

The protein

Catalyzed reaction/ biological activity

• selectively modifies Cys22 in a 56-amino acid peptide substrate [protein|1A6D90298D039FFFD977B2534952BA5E32B3530F|SunA] and can accept a variety of NDP-sugars [Pubmed|21196935]

Protein family

• [SW|glycosyltransferase 2 family] (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|1A6D90298D039FFFD977B2534952BA5E32B3530F|sunA]-[gene|260693EE7F5B5CE2A141149D292405E84B7A5FF4|sunT]-[gene|DB5F719C0A2F610301E174F6890DA9E826C76932|bdbA]-[gene|6B2DC9873B7B693D9D0FC0D773740140D0640C9E|yolJ]-[gene|12CE5AE0BD02C989E803480A1CE5A47D6192D751|bdbB]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|15743949], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]
  • [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh]: activation, [Pubmed|20817675,19465659], in [regulon|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|27902860,15743949], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]
  • [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb]: activation, [Pubmed|16306698], in [regulon|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675,17720793], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]-[SW|DnaA] [Pubmed|27902860,15743949]
  • expression starts in the stationary phase [pubmed|30808982]
  • expression is heterogeneous in the population, this is mediated by [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB], [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok], and [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A] [pubmed|30808982]

  additional information

  • the mRNA is very stable (> 15 min) [pubmed|12884008]
  • the amount of the mRNA is substantially decreased upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• BKE21450 (Δ[gene|6B2DC9873B7B693D9D0FC0D773740140D0640C9E|yolJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCTATCACCATTTTT, downstream forward: _UP4_GTATGAATACAAGATATGTA
• BKK21450 (Δ[gene|6B2DC9873B7B693D9D0FC0D773740140D0640C9E|yolJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCTATCACCATTTTT, downstream forward: _UP4_GTATGAATACAAGATATGTA

References

• 27902860,15743949,21196935,20817675,21910430,21815947