remA [2019-07-01 10:29:30]

transcriptional regulator of the extracellular matrix genes, acts in parallel to SinR, AbrB, and DegU

locus: BSU15670

pI: 7.18

mw: 9.71 kDa

protein length: 89 aa

gene length: 270 bp

function: control of biofilm formation

product: regulator of the extracellular matrix genes

essential: no

synonyms: ylzA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]

Gene

Coordinates: 1,641,672 1,641,941

The protein

Catalyzed reaction/ biological activity

• binds opuAA, tapA, and epsA promoter regions (upstream of the actual promoter) to activate transcription [Pubmed|23646920]
• binds DNA with high co-operativity due to low conserrvation of binding sites [Pubmed|23646920]

Protein family

• UPF0296 family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

  description

  • [pubmed|22383849]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|23396918], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed during vegetative growth and early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|23396918]

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|yloI]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

  description

  • [pubmed|22383849]

• genes: [gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]-[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

  description

  • [pubmed|22383849]

  regulation

  • expressed during sporulation in the mother cell [pubmed|12161109]

Biological materials

Mutant

• BKE15670 ([gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTACGTTCCCCCTG, downstream forward: _UP4_GATGAAGGGCAGGGGTAATT
• BKK15670 ([gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTACGTTCCCCCTG, downstream forward: _UP4_GATGAAGGGCAGGGGTAATT

labs

• [SW|Daniel Kearns], Indiana University, Bloomington, USA, [http://www.bio.indiana.edu/facultyresearch/faculty/Kearns.html homepage]

References

Reviews

• 24988880

Original publications

• 19363116,22329926,23123912,23396918,23646920,25035996