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pycA [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]
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pycA [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]

pyruvate carboxylase
locus
BSU14860
pI
5.00
mw
127.00 kDa
function
replenishment of the oxaloacetate pool
product
pyruvate carboxylase
essential
no
synonyms
ylaP
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|stringent response], [SW|TnrA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,554,185 -> 1,557,631

    The protein

    [SW|Cofactors]

  • biotin [Pubmed|24816803]

    • Structure

  • [PDB|3BG5] (''S. aureus'')

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • cytoplasm (homogeneously distributed throughout the cell) [Pubmed|24825009]

    • [SW|Interactions]

  • [SW|BirA]-[SW|PycA] [Pubmed|24816803]

    • Additional information

  • PycA binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [SW|SPINE].
  • PycA is subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

    • Expression and Regulation

    Operon

  • ''[protein|search|pycA]'' [Pubmed|20081037]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|20081037]

    • Regulation

  • subject to positive stringent control upon lysine starvation [Pubmed|20081037]
  • [protein|search|TnrA]: transcription repression [Pubmed|25755103]

    • Regulatory mechanism

  • stringent response: due to presence of adenines at 1 and 2 positions of the transcript [Pubmed|20081037]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

    • Biological materials

    Mutant

  • GP793 (cat), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP1289 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

    • References

    Reviews

  • 18613815,12769720,10229653,9597748,7780827

    • Original publications

  • 18763711,20081037,24825009,25755103,24816803