SubtiBank SubtiBank
pycA
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pycA

pyruvate carboxylase
Locus
BSU_14860
Isoelectric point
5.41
Molecular weight
127.72 kDa
Protein length
1148 aa Sequence Blast
Gene length
3447 bp Sequence Blast
Function
replenishment of the oxaloacetate pool
Product
pyruvate carboxylase
Essential
no
Synonyms
ylaP

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,554,185 1,557,631

The protein

Catalyzed reaction/ biological activity

  • ATP + hydrogencarbonate + pyruvate --> ADP + H+ + oxaloacetate + phosphate (according to UniProt)
  • Domains

  • ATP-grasp doamin (aa 125-321) (according to UniProt)
  • Biotin carboxylation domain (aa 1-457) (according to UniProt)
  • Pyruvate carboxyltransferase domain (aa 534-802) (according to UniProt)
  • Biotinyl-binding domain (aa 1071-1146) (according to UniProt)
  • Cofactors

  • biotin PubMed
  • Structure

  • 3BG5 (S. aureus) PubMed
  • Localization

  • membrane associated PubMed
  • cytoplasm (homogeneously distributed throughout the cell) PubMed
  • Additional information

  • PycA binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by SPINE.
  • PycA is subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • YofA: activation, PubMed, in YofA regulon
  • stringent response: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript PubMed, in stringent response
  • TnrA: repression, in TnrA regulon
  • Regulation

  • constitutively expressed PubMed
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    Genes
    Description

    Regulation

  • subject to positive stringent control upon lysine starvation PubMed
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    Biological materials

    Mutant

  • MGNA-A544 (ylaP::erm), available at the NBRP B. subtilis, Japan
  • GP793 (cat), available in Jörg Stülke's lab
  • BKE14860 (pycA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACTTTTCTCCCCCTTAC, downstream forward: _UP4_TAAAAAACAAAGAGTGTATA
  • BKK14860 (pycA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACTTTTCTCCCCCTTAC, downstream forward: _UP4_TAAAAAACAAAGAGTGTATA
  • Expression vectors

  • pGP1289 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab
  • References

    Reviews

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    Original publications

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