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pycA
Molecular weight
127.72 kDa
Function
replenishment of the oxaloacetate pool
Product
pyruvate carboxylase
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
1,554,185 1,557,631
The protein
Catalyzed reaction/ biological activity
ATP + hydrogencarbonate + pyruvate --> ADP + H+ + oxaloacetate + phosphate (according to UniProt)Domains
ATP-grasp doamin (aa 125-321) (according to UniProt)Biotin carboxylation domain (aa 1-457) (according to UniProt)Pyruvate carboxyltransferase domain (aa 534-802) (according to UniProt)Biotinyl-binding domain (aa 1071-1146) (according to UniProt) Cofactors
Structure
Localization
membrane associated PubMedcytoplasm (homogeneously distributed throughout the cell) PubMed Additional information
PycA binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by SPINE.PycA is subject to Clp-dependent proteolysis upon glucose starvation PubMed Expression and Regulation
Biological materials
Mutant
MGNA-A544 (ylaP::erm), available at the NBRP B. subtilis, JapanGP793 (cat), available in Jörg Stülke's labBKE14860 (pycA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACTTTTCTCCCCCTTAC, downstream forward: _UP4_TAAAAAACAAAGAGTGTATABKK14860 (pycA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACTTTTCTCCCCCTTAC, downstream forward: _UP4_TAAAAAACAAAGAGTGTATA Expression vectors
pGP1289 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab References
Reviews
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Original publications
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